Use of the 16S-23S rDNA internal transcribed spacer of Roseovarius crassostreae for epizootiological studies of juvenile oyster disease (JOD)
| Autor: | Bruce J. Barber, Katherine J. Boettcher, Aaron P. Maloy |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Oyster
Genotype Molecular Sequence Data Population New York Zoology Oyster farming Aquatic Science RNA Ribosomal 16S biology.animal DNA Ribosomal Spacer medicine Animals Crassostrea Maine Rhodobacteraceae Internal transcribed spacer education Phylogeny Ecology Evolution Behavior and Systematics Epizootic education.field_of_study Base Sequence Geography biology Pigmentation Ecology biology.organism_classification medicine.disease Ostreidae RNA Ribosomal 23S Massachusetts |
| Zdroj: | Diseases of Aquatic Organisms. 76:151-161 |
| ISSN: | 1616-1580 0177-5103 |
| DOI: | 10.3354/dao076151 |
| Popis: | Juvenile oyster disease (JOD) in Crassostrea virginica is caused by the marine bacterium Roseovarius crassostreae. Although the 16S rRNA genes of the bacterial isolates exhibit little variation, 2 genetic signatures (GSI and GSII) may be discerned by Ava I digestion of the 16S-23S internal transcribed spacer (ITS). In this study we analyzed isolates from JOD epizootics throughout the northeastern USA (including affected adults for the first time) to better understand how oyster populations encounter and become affected by the pathogen. Isolates from a given epizootic usually had the same ITS signature; however, the involvement of both genetic signatures was occasionally detected, even within the same oyster. Sequencing was used to localize the variable Ava I site to a 100 bp region of low sequence identity, and detection of additional base changes resulted in the identification of 11 distinct genotypes. One genotype was found only in Martha's Vineyard, Massachusetts, USA and persisted in JOD survivors. Two genotypes were associated with Maine epizootics, and both were believed to be unique to that region until 2004, when one was detected in Martha's Vineyard among oysters that had survived colonization by the local genotype. Apparent competition between those 2 genotypes was also detected among a population of juveniles. Five genotypes were found only in New York, and the other 3 were isolated from both New York and from around Cape Cod, Massachusetts. Relationships between the geographic occurrence and phylogenetic relatedness of genotypes were compared with regional current patterns to identify possible mechanisms controlling their distribution. |
| Databáze: | OpenAIRE |
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