Effects of the loss of mismatch repair genes on single-strand annealing between divergent sequences in Saccharomyces cerevisiae
| Autor: | Ye-Seul Lim, Sung-Ho Bae, Ju-Hee Choi, Min-Ku Kim, Kyu-Jin Ahn |
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| Rok vydání: | 2021 |
| Předmět: |
Monosaccharide Transport Proteins
Base Pair Mismatch Saccharomyces cerevisiae Genes Fungal Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology DNA Mismatch Repair law.invention Fungal Proteins 03 medical and health sciences law medicine DNA Fungal Gene 030304 developmental biology Genetics Recombination Genetic 0303 health sciences Mutation 030306 microbiology Nucleic Acid Heteroduplexes General Medicine biology.organism_classification DNA-Binding Proteins MSH3 MSH2 Recombinant DNA DNA mismatch repair Heteroduplex |
| Zdroj: | Journal of microbiology (Seoul, Korea). 59(4) |
| ISSN: | 1976-3794 |
| Popis: | Eukaryotic genomes contain many duplicated genes closely located with each other, such as the hexose transporter (HXT) genes in Saccharomyces cerevisiae. They can potentially recombine via single-strand annealing (SSA) pathway. SSA between highly divergent sequences generates heteroduplex DNA intermediates with many mismatches, which can be corrected by mismatch repair (MMR), resulting in recombinant sequences with a single junction point. In this report, we demonstrate that SSA between HXT1 and HXT4 genes in MMR-deficient yeast cells produces recombinant genes with multiple-junctions resulting from alternating HXT1 and HXT4 tracts. The mutations in MMR genes had differential effects on SSA frequencies; msh6Δ mutation significantly stimulated SSA events, whereas msh2Δ and msh3Δ slightly suppressed it. We set up an assay that can identify a pair of recombinant genes derived from a single heteroduplex DNA. As a result, the recombinant genes with multiple-junctions were found to accompany genes with single-junctions. Based on the results presented here, a model was proposed to generate multiple-junctions in SSA pathway involving an alternative short-patch repair system. |
| Databáze: | OpenAIRE |
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