Characterization of Variable-Region Genes and Shared Crossreactive Idiotypes of Antibodies Specific for Antigens of Various Influenza Viruses
| Autor: | Jerome L. Schulman, Marc Monestier, Constantin A. Bona, Mary Ann Thompson, Roy Riblet, Michael A. Reale, Thomas M. Moran, Gerard P. Norton, Alexander C.K. Lai |
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| Rok vydání: | 1987 |
| Předmět: |
Idiotype
medicine.drug_class Blotting Western Immunology Orthomyxoviridae Dose-Response Relationship Immunologic Immunoglobulin Variable Region Radioimmunoassay Hemagglutinins Viral Hemagglutinin (influenza) Enzyme-Linked Immunosorbent Assay Cross Reactions Monoclonal antibody Mice Immunoglobulin Idiotypes Antigen Virology medicine Animals Antigens Viral Mice Inbred BALB C Hybridomas Genes Immunoglobulin biology Antibodies Monoclonal biology.organism_classification Molecular biology biology.protein Molecular Medicine Antibody Neuraminidase |
| Zdroj: | Viral Immunology. 1:1-12 |
| ISSN: | 1557-8976 0882-8245 |
| DOI: | 10.1089/vim.1987.1.1 |
| Popis: | Several syngeneic monoclonal anti-idiotypic antibodies were obtained against PY206, a monoclonal antibody specific for X-31 (H3N2) influenza virus hemagglutinin. This idiotype was found in the sera of BALB/c mice immunized with various influenza viruses. Adsorption experiments indicated that the PY206 Id was borne by antibodies specific for viral hemagglutinin (HA) and/or neuraminidase (NA). This idiotype was identified on other monoclonal antibodies specific for various influenza HAs (H3 and H1). Study of the variable-region (V) genes of these monoclonal antibodies showed that its expression is independent of variable kappa (Vk)21 light-chains and that the heavy-chains of the strongly idiotype-positive hybridomas derive from either the variable heavy (VH) J558 or VH 7183 family. Finally, Western blot analysis demonstrated that PY206 idiotypic determinants are located exclusively on the heavy chain. |
| Databáze: | OpenAIRE |
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