Direct Comparison of Therapeutic Effects on Diabetic Polyneuropathy between Transplantation of Dental Pulp Stem Cells and Administration of Dental Pulp Stem Cell-Secreted Factors

Autor: Taisuke Yamauchi, Eriko Makino, Megumi Miyabe, Shigemi Goto, Shun Kondo, Tomomi Minato, Nobuhisa Nakamura, Keiko Naruse, Saki Kanada, Ken Miyazawa, Tatsuaki Matsubara, Mizuho Ito, Tomokazu Saiki, Noritaka Sawada, Masaki Hata
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Blood Glucose
Male
Pathology
Neural Conduction
Hindlimb
Nerve conduction velocity
dental pulp stem cell
lcsh:Chemistry
Rats
Sprague-Dawley
0302 clinical medicine
Nerve Fibers
diabetic polyneuropathy
Diabetic Neuropathies
Medicine
lcsh:QH301-705.5
Spectroscopy
Motor Neurons
Stem Cells
General Medicine
Sciatic Nerve
Computer Science Applications
medicine.anatomical_structure
Sciatic nerve
Stem cell
medicine.drug
medicine.medical_specialty
regenerative medicine
Nerve fiber
secreted factor
Catalysis
Article
Diabetes Mellitus
Experimental
Inorganic Chemistry
03 medical and health sciences
stomatognathic system
Dental pulp stem cells
Animals
Nerve Growth Factors
Physical and Theoretical Chemistry
Muscle
Skeletal
Molecular Biology
Dental Pulp
business.industry
Organic Chemistry
Body Weight
Streptozotocin
Transplantation
stomatognathic diseases
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Culture Media
Conditioned
business
030217 neurology & neurosurgery
Stem Cell Transplantation
transplantation
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 17
International Journal of Molecular Sciences, Vol 21, Iss 6064, p 6064 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21176064
Popis: Stem cell transplantation is a potential novel therapy for diabetic polyneuropathy. Dental pulp stem cells (DPSCs) are attractive stem cell sources because DPSCs can be isolated from extracted teeth and cryopreserved while retaining viability. In this study, we directly compared the efficacy of the transplantation of DPSCs and the administration of the secreted factors from DPSCs (DPSC-SFs) on diabetic polyneuropathy. Eight weeks after streptozotocin injection, DPSCs (1.0 ×
106 cells/rat) or DPSC-SFs (1.0 mL/rat) were administered into the unilateral hindlimb skeletal muscles of diabetic Sprague&ndash
Dawley rats. DPSC transplantation and DPSC-SF administration did not affect blood glucose levels and body weights in the diabetic rats. Both DPSC transplantation and DPSC-SF administration significantly ameliorated sciatic nerve conduction velocity and sciatic nerve blood flow, accompanied by increases in muscle bundle size, vascular density in the skeletal muscles and intraepidermal nerve fiber density in the diabetic rats, while there was no difference between the results for DPSCs and DPSC-SFs. These results suggest that the efficacy of both DPSC transplantation and DPSC-SF administration for diabetic polyneuropathy four weeks after transplantation/administration was mainly due to the multiple secretomes secreted from transplanted DPSCs or directly injected DPSC-SFs in the early phase of transplantation/administration.
Databáze: OpenAIRE
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