Inhalation of water electrolysis-derived hydrogen ameliorates cerebral ischemia–reperfusion injury in rats – A possible new hydrogen resource for clinical use
| Autor: | Xiaoqun Li, Xuejun Sun, Jun Zhang, Zhengrong Gu, Rongjia Zhang, Jiacan Su, Xiao Zhai, Jiong Hou, Fang Ji, Xin Zhi, Weizong Weng, Xiao Chen, Jin Cui, Dongchen Shi, Liehu Cao, Liping Wang |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Hydrogen Ischemia chemistry.chemical_element Apoptosis Pharmacology medicine.disease_cause Neuroprotection Brain Ischemia Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine Animals Neurons chemistry.chemical_classification Reactive oxygen species TUNEL assay Inhalation Chemistry General Neuroscience Brain Water medicine.disease Neuroprotective Agents 030104 developmental biology Reperfusion Injury Reperfusion injury 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Neuroscience. 335:232-241 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2016.08.021 |
| Popis: | Hydrogen is a kind of noble gas with the character to selectively neutralize reactive oxygen species. Former researches proved that low-concentration of hydrogen can be used to ameliorating cerebral ischemia/reperfusion injury. Hydrogen electrolyzed from water has a hydrogen concentration of 66.7%, which is much higher than that used in previous studies. And water electrolysis is a potential new hydrogen resource for regular clinical use. This study was designed and carried out for the determination of safety and neuroprotective effects of water electrolysis-derived hydrogen. Sprague-Dawley rats were used as experimental animals, and middle cerebral artery occlusion was used to make cerebral ischemia/reperfusion model. Pathologically, tissues from rats in hydrogen inhalation group showed no significant difference compared with the control group in HE staining pictures. The blood biochemical findings matched the HE staining result. TTC, Nissl, and TUNEL staining showed the significant improvement of infarction volume, neuron morphology, and neuron apoptosis in rat with hydrogen treatment. Biochemically, hydrogen inhalation decreased brain caspase-3, 3-nitrotyrosine and 8-hydroxy-2-deoxyguanosine-positive cells and inflammation factors concentration. Water electrolysis-derived hydrogen inhalation had neuroprotective effects on cerebral ischemia/reperfusion injury in rats with the effect of suppressing oxidative stress and inflammation, and it is a possible new hydrogen resource to electrolyze water at the bedside clinically. |
| Databáze: | OpenAIRE |
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