Development and Validation of a Novel, Rapid Gradient HPLC Method for Simultaneous Estimation of Bioactive Marker Compounds in a Mixture of Convolvulus pluricaulis, Withania somnifera and Bacopa monnieri Extracts

Autor:	Indu Pal Kaur, Jai Malik, Sunayna Choudhary
Rok vydání:	2019
Předmět:	Analyte Convolvulus Convolvulus pluricaulis Withania somnifera Withania 01 natural sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Limit of Detection Scopoletin Bacopa monnieri Bacoside A Withanolides Chromatography High Pressure Liquid Detection limit Chromatography biology Plant Extracts 010401 analytical chemistry Reproducibility of Results General Medicine biology.organism_classification Triterpenes 0104 chemical sciences chemistry Withaferin A Linear Models Bacopa 030217 neurology & neurosurgery Biomarkers
Zdroj:	Journal of chromatographic science. 57(10)
ISSN:	1945-239X
Popis:	The use of herbs as medicine is an ancient form of healthcare known to mankind. Standardization of herbal medicines is however a challenging task and is the major bottleneck in their acceptance as the primary therapeutic option. The aim of this study was to develop and validate a simple, rapid HPLC method for standardizing the mixture of extracts of three Medhya Rasayanas (neurotonic), Convolvulus pluricaulis, Withania somnifera and Bacopa monnieri. Simultaneous estimation of the respective bioactive markers of these plants viz., scopoletin, withaferin A, bacoside A 3, bacopaside II, jujubogenin and bacosaponin C has been reported for the first time. The method was developed using Waters Hybrid X-Bridge shield with BEH technology 2.5 μm, 4.6 × 75 mm column and validated according to ICH guidelines. The 20 minutes run time makes the method eco-friendly. The method was linear over a range of 12.5–400 ng/10 μL for scopoletin and 62.5–2,000 ng/10 μL for withaferin A, bacoside A 3, bacopaside II, jujubogenin and bacosaponin C with detection limits of 8.0, 48.3, 30.4, 40.7, 15.6 and 18.9 ng/10 μL and quantification limits of 24.5, 146.5, 92.2, 123.4, 47.4 and 57.4 ng/10 μL, respectively. The correlation coefficient for each analyte was >0.999. The intra-day and inter-day precision was
Databáze:	OpenAIRE
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