Susceptibility and resistance to Echinococcus granulosus infection: Associations between mouse strains and early peritoneal immune responses
| Autor: | Rafael Capurro, Gustavo Mourglia-Ettlin, Alicia Merlino, Sylvia Dematteis |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
C57BL/6 030231 tropical medicine Immunology BALB/c Mice 03 medical and health sciences 0302 clinical medicine Immune system Species Specificity Echinococcosis Immunity parasitic diseases Animals Immunology and Allergy Echinococcus granulosus Antibody-dependent cell-mediated cytotoxicity Mice Inbred BALB C Principal Component Analysis biology Hematology biology.organism_classification In vitro Mice Inbred C57BL 030104 developmental biology Cytokines Cattle Tumor necrosis factor alpha Disease Susceptibility Peritoneum Biomarkers |
| Zdroj: | Immunobiology. 221:418-426 |
| ISSN: | 0171-2985 |
| DOI: | 10.1016/j.imbio.2015.11.012 |
| Popis: | In helminth infections, there are no easy associations between host susceptibility and immune responses. Interestingly, immunity to cestodes - unlike most helminths - seems to require Th1-type effectors. In this sense, we reported recently that Balb/c and C57Bl/6 mice are high and low susceptible strains, respectively, to experimental infection by Echinococcus granulosus. However, the role of the early cellular peritoneal response in such differential susceptibility is unknown. Here, we analyzed the kinetics of cytokines expression and cellular phenotypes in peritoneal cells from infected Balb/c and C57Bl/6 mice. Additionally, Principal Components Analysis (PCA) were conducted to highlight the most relevant differences between strains. Finally, the anti-parasite activities of peritoneal cells were assessed through in vitro systems. PCAs clustered C57Bl/6 mice by their early mixed IL-5/TNF-α responses and less intense expression of Th2-type cytokines. Moreover, they exhibited lower counts of eosinophils and higher numbers of macrophages and B cells. Functional studies showed that peritoneal cells from infected C57Bl/6 mice displayed greater anti-parasite activities, in accordance with higher rates of NO production and more efficient ADCC responses. In conclusion, mild Th2-responses and active cellular mechanisms are key determinants in murine resistance to E. granulosus infection, supporting the cestode immune exception among helminth parasites. |
| Databáze: | OpenAIRE |
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