Lymphocyte subsets and cellular immunity in patients with chronic pancreatitis
| Autor: | R J Benschop, R E Schmidt, Johann Ockenga, R. Jacobs, A. Kemper, M.P. Manns |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Cellular immunity Pancreatic disease Pain Biology Pathogenesis Immune system Immunity Immunopathology medicine Humans skin and connective tissue diseases Immunity Cellular Monocyte Gastroenterology Middle Aged medicine.disease Flow Cytometry Lymphocyte Subsets medicine.anatomical_structure Phenotype Pancreatitis Immunology Chronic Disease Female sense organs |
| Zdroj: | Digestion. 62(1) |
| ISSN: | 0012-2823 |
| Popis: | Background: An abnormal immune response may play a pathogenetic role in chronic pancreatitis. However, to date characterization of the systemic immunological changes in patients with chronic pancreatitis has not been undertaken. Methods: Lymphocyte phenotypes and proliferation (3H-thymidine) after stimulation with mitogens and interleukin-2 were studied in peripheral mononuclear cells from 11 patients with chronic pancreatitis (alcohol-induced n = 6; idiopathic pancreatitis n = 5). The natural killer cell activity was investigated in a 51Cr release cytotoxicity assay. In vitro cytokine release of stimulated mononuclear cells was measured. Results: Flow cytometric studies showed a significant decrease in the percentage of circulating CD8+, CD56+ and CD25+ cells in patients with chronic pancreatitis independent of the etiology. Comparing all patients with chronic pancreatitis to controls, the proliferation rate was not significantly increased, but patients with pain (n = 5) showed increased proliferation in comparison to patients without pain (n = 6). No significant difference in natural killer cytotoxicity was observed. The in vitro release of tumor necrosis factor-α and interleukin-10 by stimulated mononuclear cells was increased. Conclusions: Chronic pancreatitis is accompanied by systemic immune dysregulation. The observed changes in peripheral mononuclear cells reveal additional evidence that the cell-mediated immune response may contribute to the development of pain and tissue destruction in chronic pancreatitis. |
| Databáze: | OpenAIRE |
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