The Cysteine-Rich Peptide Snakin-2 Negatively Regulates Tubers Sprouting through Modulating Lignin Biosynthesis and H2O2 Accumulation in Potato
| Autor: | Cai Chengcheng, Liqin Li, Huang Xueli, Deng Mengsheng, Xi-Yao Wang, Su Ni, Yu Liping, Ran Shuang, Peng Jie, Zhang Jie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine dormancy Snakin-2 potato tuber Cinnamyl-alcohol dehydrogenase lignin hydrogen peroxide 01 natural sciences Catalysis Inorganic Chemistry Superoxide dismutase lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound Caffeic acid Lignin Physical and Theoretical Chemistry Molecular Biology Gibberellic acid lcsh:QH301-705.5 Spectroscopy biology Chemistry Organic Chemistry fungi food and beverages General Medicine sprouting Computer Science Applications 030104 developmental biology Biochemistry lcsh:Biology (General) lcsh:QD1-999 Catalase biology.protein Dormancy 010606 plant biology & botany Peroxidase |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 2287, p 2287 (2021) International Journal of Molecular Sciences Volume 22 Issue 5 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Potato tuber dormancy is critical for the post-harvest quality. Snakin/Gibberellic Acid Stimulated in Arabidopsis (GASA) family genes are involved in the plants’ defense against pathogens and in growth and development, but the effect of Snakin-2 (SN2) on tuber dormancy and sprouting is largely unknown. In this study, a transgenic approach was applied to manipulate the expression level of SN2 in tubers, and it demonstrated that StSN2 significantly controlled tuber sprouting, and silencing StSN2 resulted in a release of dormancy and overexpressing tubers showed a longer dormant period than that of the control. Further analyses revealed that the decrease expression level accelerated skin cracking and water loss. Metabolite analyses revealed that StSN2 significantly down-regulated the accumulation of lignin precursors in the periderm, and the change of lignin content was documented, a finding which was consistent with the precursors’ level. Subsequently, proteomics found that cinnamyl alcohol dehydrogenase (CAD), caffeic acid O-methyltransferase (COMT) and peroxidase (Prx), the key proteins for lignin synthesis, were significantly up-regulated in silencing lines, and gene expression and enzyme activity analyses also supported this effect. Interestingly, we found that StSN2 physically interacts with three peroxidases catalyzing the oxidation and polymerization of lignin. In addition, SN2 altered the hydrogen peroxide (H2O2) content and the activities of superoxide dismutase (SOD) and catalase (CAT). These results suggest that StSN2 negatively regulates lignin biosynthesis and H2O2 accumulation, and ultimately inhibits the sprouting of potato tubers. |
| Databáze: | OpenAIRE |
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