The Combined Effect of Melatonin Implant and Removal of Buck Seminal Plasma on Cryopreservation During the Nonbreeding Season

Autor:	Burcu Ustuner, Hakan Ustuner, Elif Gokce, N. Tekin Onder, M. Melih Yilmaz, Oguzhan Huraydin, M. Berk Toker
Rok vydání:	2022
Předmět:	Medicine (miscellaneous) Cell Biology General Medicine General Biochemistry Genetics and Molecular Biology
Zdroj:	Biopreservation and Biobanking.
ISSN:	1947-5543 1947-5535
DOI:	10.1089/bio.2022.0074
Popis:	This study aimed to determine how melatonin (MT) and seminal plasma affected the freezability of buck sperm during the nonbreeding season. Semen was collected from eight bucks before (pre-MT) and after (post-MT) MT application in the nonbreeding season. Individual ejaculates were collected from the bucks, split into two equal groups according to the removal of seminal plasma (SP) (-) or nonremoval of SP (+). For washing, the groups of ejaculates were centrifuged, and the supernatant was separated, SP (-) and SP (+) ejaculates were diluted, then frozen. Semen samples were examined for sperm motility, plasma membrane integrity, defective acrosomes, DNA fragmentation, and mitochondrial membrane function at the native and post-thaw stages. When the general average post-thaw motility (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9841f3ad5191c7eea0de3029533bf1d3 https://doi.org/10.1089/bio.2022.0074 Zobrazit plný text záznamu