A new self-compatibility haplotype in the sweet cherry 'Kronio', S5' attributable to a pollen-part mutation in the SFB gene
| Autor: | Annalisa Marchese, Marcello Cutuli, Antonio Raimondo, Radovan I. Bošković, Tiziano Caruso, Kenneth R. Tobutt |
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| Přispěvatelé: | MARCHESE A, BOSKOVIC RI, CARUSO T, RAIMONDO A, CUTULI M, TOBUTT KR |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Physiology
Molecular Sequence Data Plant Science Flowers Biology Polymerase Chain Reaction Prunus Ribonucleases Chromosome Segregation Genotype Amino Acid Sequence Pollination Gene Alleles Crosses Genetic Genetics F-Box Proteins Haplotype Intron food and beverages Selfing Sequence Analysis DNA Haplotypes Seedlings Mutation Microsatellite Ploidy |
| Popis: | ‘Kronio’ is a Sicilian cultivar of sweet cherry (Prunus avium), nominally with the incompatibility genotype S 5 S 6 , that is reported to be naturally self-compatible. In this work the cause of its self-compatibility was investigated. Test selfing confirmed self-compatibility and provided embryos for analysis; PCR with consensus primers designed to amplify S-RNase and SFB alleles showed that the embryos were of two types, S 5 S 5 and S 5 S 6 , indicating that S 6 pollen failed, but S 5 succeeded, perhaps because of a mutation in the pollen or stylar component. Stylar RNase analysis indicated active S-RNases for both S 5 and S 6 . The S-RNase alleles were cloned and sequenced; and sequences encode functional proteins. Cloning and sequencing of SFB alleles showed that S 6 was normal but S 5 had a premature stop codon upstream of the variable region HVa resulting in a truncated protein. Therefore, the self-compatibility can be attributed to a pollen-part mutation of S 5 , designated S 5 ′, the first reported case of breakdown of self-incompatibility in diploid sweet cherry caused by a natural mutation at the S-locus. The second intron of the S-RNase associated with S 5 ′ contained a microsatellite smaller than that associated with S 5 ; primers designed to amplify across this microsatellite effectively distinguished S 5 from S 5 ′. Analysis of some other Sicilian cherries with these primers indicated that S 5 ′ is also present in the Sicilian cultivar ‘Maiolina a Rappu’, and this proved to be self-compatible. |
| Databáze: | OpenAIRE |
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