Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12
| Autor: | Garry Blakely, Susan T. Lovett, Lidia K. Arciszewska, Mary E. Burke, David J. Sherratt, Gerhard May, Richard McCulloch |
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| Rok vydání: | 1993 |
| Předmět: |
Recombination
Genetic Genetics Base Sequence Integrases Sequence Homology Amino Acid Escherichia coli Proteins FLP-FRT recombination Molecular Sequence Data Cre recombinase Biology General Biochemistry Genetics and Molecular Biology Substrate Specificity Recombinases Multigene Family DNA Nucleotidyltransferases Escherichia coli Recombinase Amino Acid Sequence Site-specific recombination Cre-Lox recombination Homologous recombination Recombination Floxing Plasmids |
| Zdroj: | Cell. 75:351-361 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(93)80076-q |
| Popis: | The stable inheritance of ColE1-related plasmids and the normal partition of the E. coli chromosome require the function of the Xer site-specific recombination system. We show that in addition to the XerC recombinase, whose function has already been implicated in this system, a second chromosomally encoded recombinase, XerD, is required. The XerC and XerD proteins show 37% identity and bind to separate halves of the recombination site. Both proteins act catalytically in the recombination reaction. Recombination site asymmetry and the requirement of two recombinases ensure that only correctly aligned sites are recombined. We predict that normal partition of most circular chromosomes requires the participation of site-specific recombination to convert any multimers (arising by homologous recombination) to monomers. |
| Databáze: | OpenAIRE |
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