Differences in epidermal thickness and expression of apoptosis regulatory proteins in the skin of patients with chronic renal failure and pruritus
| Autor: | Lina Mirić Kovačević, Danijela Kalibovic Govorko, Snježana Mardešić Brakus, Neira Puizina-Ivić, Dragan Ljutić, Dino Mirić, Mirna Saraga-Babić, Jasminka Rešić, Ivo Jeličić |
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| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Programmed cell death Histology Cell Survival Biopsy medicine.medical_treatment Apoptosis chronic renal failure skin thickness pruritus apoptosis Basal (phylogenetics) Renal Dialysis In Situ Nick-End Labeling medicine Humans Dialysis Aged Skin bcl-2-Associated X Protein TUNEL assay Cell Death integumentary system Epidermis (botany) medicine.diagnostic_test Caspase 3 business.industry Pruritus Epithelial Cells Cell Biology General Medicine Middle Aged Immunohistochemistry Microscopy Fluorescence Proto-Oncogene Proteins c-bcl-2 Kidney Failure Chronic Epidermis business Free nerve ending |
| Zdroj: | Acta Histochemica. 115:144-150 |
| ISSN: | 0065-1281 |
| Popis: | Chronic renal failure is often associated with skin itching (pruritus) in dialysis patients. In order to investigate the possible causes of pruritus, the epidermis of the thigh of 12 dialysis patients and 4 controls from patients without renal disease were examined. The sections of the epidermis were measured and immunohistochemically analyzed using antibodies to Bcl-2, Bax, caspase-3 proteins and TUNEL method. While the mean thickness of normal epidermis was 53 pm, in dialysis patients it ranged between 23 and 34 mu m during the 3-5 year period on dialysis. Compared to normal skin, the fine balance between the Bcl-2 and Bax proteins did not greatly change in the epidermis of dialysis patients during the three years of dialysis. Following five-year dialysis, the epidermis displayed increased Bax and decreased Bcl-2 expression in the basal and intermediate epidermal layers, as well as the presence of apoptotic cells (TUNEL and caspase-3 positive) both in the superficial and intermediate epidermal layers. Our study demonstrated the predominant expression of cell death Bax proteins over cell survival Bcl-2 proteins, and apoptotic cells in the deeper layers of the epidermis in patients on long-term dialysis. We speculate that the thinning of the epidermis might be associated with the appearance of dead cells in the deeper epidermal layers, while the changed internal milieu of epidermal cells could possibly affect the intra-epidermal nerve endings thus leading to the sensation of pruritus. |
| Databáze: | OpenAIRE |
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