Genetic Modification of Low Phytic Acid 1-1 Maize to Enhance Iron Content and Bioavailability
| Autor: | Steve Rodermel, Maneesha Aluru, Manju B. Reddy |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Phytic acid
Genetically modified maize Phytic Acid biology Iron Biofortification Biological Availability food and beverages General Chemistry Genetically modified crops Plants Genetically Modified biology.organism_classification Zea mays Bioavailability Ferritin chemistry.chemical_compound Agronomy chemistry Seedling Germination Food Fortified Seeds biology.protein Food science General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 59:12954-12962 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf203485a |
| Popis: | High phytate content in staple food crops is a major barrier to successful iron biofortification. We have exploited the low phytic acid 1-1 (lpa1-1) mutant of maize to generate transgenic plants with up-to 70 μg/g seed iron through the endosperm-specific overexpression of soybean ferritin, resulting in more than 2-fold improvement in iron bioavailability. The levels of bioavailable seed iron achieved in this study greatly exceed any achieved thus far and closely approach values estimated to have a nutritional impact on target populations. Gene expression studies reveal a large induction of the YS1 transporter in leaves and severe repression of an iron acquisition gene DMAS1 in roots, suggesting significant alterations in the iron homeostatic mechanisms in transgenic lpa1-1. Furthermore, preliminary tests show that the high-iron lpa1-1 seeds have higher germination rates and seedling vigor when compared to those of the nontransgenic seeds, which may help improve their value to plant breeders. |
| Databáze: | OpenAIRE |
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