Compound C inhibits hypoxic activation of HIF-1 independent of AMPK
| Autor: | Navdeep S. Chandel, Kathryn V. Tormos, Brooke M. Emerling, Benoit Viollet |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
AMP-activated kinase
Biophysics Regulator Repressor Biology Mitochondrion AMP-Activated Protein Kinases Protein Serine-Threonine Kinases Biochemistry Article AMP-activated protein kinase Structural Biology Multienzyme Complexes Genetics medicine Hypoxia Molecular Biology Transcription factor Protein Kinase Inhibitors Hypoxia inducible factor 1 Kinase AMPK Cell Biology Hypoxia (medical) Cell Hypoxia Cell biology Mitochondria Pyrimidines biology.protein Pyrazoles Hypoxia-Inducible Factor 1 medicine.symptom Reactive Oxygen Species Compound C |
| Zdroj: | FEBS letters. 581(29) |
| ISSN: | 0014-5793 |
| Popis: | The key transcription factor that regulates the cellular responses to hypoxia is hypoxia inducible factor-1 (HIF-1). The signaling mechanisms that regulate the hypoxic activation of HIF-1 are not fully understood. Our objective here was to test whether AMP-activated kinase (AMPK) was an upstream regulator of HIF-1. Our results show that AMPK is not required for the hypoxic activation of HIF-1. Interestingly, the AMPK inhibitor, Compound C, inhibits the hypoxic activation of HIF-1 independent of AMPK. Furthermore, we demonstrate that Compound C functions as a repressor of HIF-1 by inhibiting respiration and suppressing mitochondrial generated ROS. |
| Databáze: | OpenAIRE |
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