Low Levels of IgG Antibodies Against Pneumocystis carinii Among HIV-infected Patients

Autor: Alex Lund Laursen, Paul L. Andersen
Rok vydání: 1998
Předmět:
Adult
Male
Microbiology (medical)
Opportunistic infection
Enzyme-Linked Immunosorbent Assay
HIV Infections
Cross Reactions
Immunoglobulin G
Serology
AIDS-Related Opportunistic Infections/immunology
HIV Infections/immunology
Antigen
Immunopathology
Antigens
Bacterial/immunology
medicine
Animals
Humans
Rats
Wistar
Immunoglobulin G/immunology
Antigens
Bacterial
Antibodies
Bacterial/immunology
AIDS-Related Opportunistic Infections
General Immunology and Microbiology
biology
Pneumocystis
Enzyme-Linked Immunosorbent Assay/methods
Pneumonia
Pneumocystis
Pneumocystis/immunology
General Medicine
Middle Aged
medicine.disease
Antibodies
Bacterial
Rats
respiratory tract diseases
Infectious Diseases
Pneumocystis carinii
Antibody Formation
Humoral immunity
Immunology
biology.protein
Female
Antibody
Pneumonia
Pneumocystis/immunology
Zdroj: Laursen, A L & Andersen, P L 1998, ' Low levels of IgG antibodies against pneumocystis carinii among HIV-infected patients ', Scandinavian Journal of Infectious Diseases, vol. 30, no. 5, pp. 495-9 . https://doi.org/10.1080/00365549850161511
ISSN: 1651-1980
0036-5548
DOI: 10.1080/00365549850161511
Popis: IgG antibodies against Pneumocystis carinii (P. carinii) were detected by an ELISA method using urea-extracted material from human and rat P. carinii as the antigen. Carbohydrate formed a major part of the antigen responsible for reactivity in the ELISA assay, since periodate treatment reduced the reactivity of most sera tested. Cross-reactivity between human and rat P. carinii was detected. However, human serum recognized antigens specific for human P. carinii. With the ELISA method IgG antibody levels were compared between blood donors (n = 40), asymptomatic HIV-antibody positive patients (n = 30) and AIDS patients with (n=22) and without previous P. carinii pneumonia (PCP) (n=21). HIV-infected patients had significantly lower antibody reactivity against the microorganism compared with blood donors. Among HIV-antibody positive patients the highest antibody reactivity was seen in PCP patients. The antibody response to PCP was impaired, since an equal number of patients had an increase and a decrease in antibody reactivity. In conclusion, carbohydrate formed an important part of the P. carinii immunogenic antigen. Cross-reactivity between rat and human P. carinii was demonstrated, but reactivity was somewhat lower using antigen from rats. The antibody level was lower in HIV-infected patients and the ability to mount an antibody response to the infection was impaired, suggesting that the poor antibody response may contribute to the liability of HIV-infected patients to have PCP.
Databáze: OpenAIRE
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