Genetic engineering of cotton with a novel cry2AX1 gene to impart insect resistance against Helicoverpa armigera
| Autor: | V. Udayasuriyan, Duraialagaraja Sudhakar, Sundararajan Sathish, Karunamurthy Dhivya, N. Balakrishnan |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:Biotechnology Transgene Genetically modified crops lcsh:Plant culture Helicoverpa armigera cry2AX1 01 natural sciences Microbiology 03 medical and health sciences lcsh:TP248.13-248.65 Bacillus thuringiensis Botany Bioassay lcsh:SB1-1110 Gene General Environmental Science Southern blot biology fungi food and beverages Agrobacterium tumefaciens somatic embryogenesis biology.organism_classification 030104 developmental biology General Earth and Planetary Sciences 010606 plant biology & botany |
| Zdroj: | Crop Breeding and Applied Biotechnology v.16 n.3 2016 Crop Breeding and Applied Biotechnology Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB Crop Breeding and Applied Biotechnology, Volume: 16, Issue: 3, Pages: 205-212, Published: SEP 2016 Crop Breeding and Applied Biotechnology, Vol 16, Iss 3, Pp 205-212 (2016) |
| ISSN: | 1984-7033 |
| DOI: | 10.1590/1984-70332016v16n3a31 |
| Popis: | Embryogenic calli of cotton (Coker310) were cocultivated with the Agrobacterium tumefaciens strain LBA4404 harbouring the codon-optimised, chimeric cry2AX1 gene consisting of sequences from cry2Aa and cry2Ac genes isolated from Indian strains of Bacillus thuringiensis. Forty-eight putative transgenic plants were regenerated, and PCR analysis of these plants revealed the presence of the cry2AX1 gene in 40 plants. Southern blot hybridisation analysis of selected transgenic plants confirmed stable T-DNA integration in the genome of transformed plants. The level of Cry2AX1 protein expression in PCR positive plants ranged from 4.9 to 187.5 ng g-1 of fresh tissue. A transgenic cotton event, TP31, expressing the cry2AX1 gene showed insecticidal activity of 56.66 per cent mortality against Helicoverpa armigera in detached leaf disc bioassay. These results indicate that the chimeric cry2AX1 gene expressed in transgenic cotton has insecticidal activity against H. armigera. |
| Databáze: | OpenAIRE |
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