Efficacy of seven retrograde tracers, compared in multiple-labelling studies of feline motoneurones
| Autor: | D.B. Thomson, E. Smits, J.L. Creasy, Rebecca A. Gladdy, Seiichiro Kitamura, F. J. R. Richmond |
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| Rok vydání: | 1994 |
| Předmět: |
Latex
Stilbamidines Amidines Gold Colloid Axonal Transport Horseradish peroxidase chemistry.chemical_compound Neck Muscles medicine Animals Fluorescein Coloring Agents Horseradish Peroxidase Fluorescent Dyes Motor Neurons biology Rhodamines General Neuroscience Dextrans Anatomy Carbocyanines Motor neuron Fluoresceins Spinal cord Molecular biology Microspheres medicine.anatomical_structure Dextran chemistry Evaluation Studies as Topic Cats biology.protein Axoplasmic transport Intramuscular injection Peroxidase |
| Zdroj: | Journal of Neuroscience Methods. 53:35-46 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/0165-0270(94)90142-2 |
| Popis: | The labelling efficacies of 7 retrograde tracers were evaluated following cut nerve exposure or intramuscular injection into the serially compartmentalized neck muscle, biventer cervicis. Tested tracers included Fast Blue (FB), Fluorogold (FG), dextran conjugated to fluorescein (FD), dextran conjugated to rhodamine (Fluororuby (FR), 3000 and 10,000 MW), fluorescent latex microspheres, horseradish peroxidase coupled to colloidal gold, and 1,1'-dioctadecyl-3,3,3',3'-tetramethyl indocarbocyanine perchlorate (DiI). In 2 animals, horseradish peroxidase was also employed and spinal cords were processed for peroxidase activity to evaluate its effect on the appearance of cells labelled with fluorescent tracers. Four tracers, FB, FG, FD and FR, could be observed in motoneurones under the conditions of our study. FB and FG labelled comparable numbers of motoneurones following cut nerve exposure, but dissimilar numbers following intramuscular injection. FG diffused extensively following injection and was found in motoneurones not only in the appropriate ipsilateral segment but also adjacent ipsilateral and contralateral segments. Intramuscular injections of FB usually labelled fewer cells than cut nerve exposure, but evidence for spurious labelling following intramuscular injection could also be found. FD or FR labelled motoneurones following cut nerve exposure but not following intramuscular injection. The conjugated dextrans labelled more variable numbers of cells than FB or FG, but the labelled cells had similar patterns of distribution. The remaining tracers were ineffective as retrograde markers in our study, and the possible reasons for these failures are discussed. |
| Databáze: | OpenAIRE |
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