Site-specific covalent labeling of His-tag fused proteins with N-acyl-N-alkyl sulfonamide reagent
Autor: | Vikram Thimaradka, Christina Heroven, A. Radu Aricescu, Itaru Hamachi, Tomonori Tamura, Jae Hoon Oh, Michisuke Yuzaki |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Models
Molecular Nitrilotriacetic Acid Clinical Biochemistry Pharmaceutical Science Peptide 01 natural sciences Biochemistry Article chemistry.chemical_compound Nucleophile Nickel Drug Discovery Humans Histidine Molecular Biology chemistry.chemical_classification Sulfonamides Molecular Structure Staining and Labeling 010405 organic chemistry Lysine Organic Chemistry Nitrilotriacetic acid Proteins Combinatorial chemistry 0104 chemical sciences Sulfonamide 010404 medicinal & biomolecular chemistry HEK293 Cells chemistry Covalent bond Molecular Probes Molecular Medicine Indicators and Reagents Molecular probe Conjugate |
Zdroj: | Bioorganic & medicinal chemistry |
ISSN: | 0968-0896 |
Popis: | The ability to incorporate a desired functionality into proteins of interest in a site-specific manner can provide powerful tools for investigating biological systems and creating therapeutic conjugates. However, there are not any universal methods that can be applied to all proteins, and it is thus important to explore the chemical strategy for protein modification. In this paper, we developed a new reactive peptide tag/probe pair system for site-specific covalent protein labeling. This method relies on the recognition-driven reaction of a peptide tag and a molecular probe, which comprises the lysine-containing short histidine tag (KH6 or H6K) and a binuclear nickel (II)- nitrilotriacetic acid (Ni2+-NTA) complex probe containing a lysine-reactive N-acyl-N-alkyl sulfonamide (NASA) group. The selective interaction of the His-tag and Ni2+-NTA propeles a rapid nucleophilic reaction between a lysine residue of the tag and the electrophilic NASA group of the probe by the proximity effect, resulting in the tag-site-specific functionalization of proteins. We characterized the reactive profile and site-specificity of this method using model peptides and proteins in vitro, and demonstrated the general utility for production of a nanobody-chemical probe conjugate without compromising its binding ability. |
Databáze: | OpenAIRE |
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