Part I. Development of a model system for studying nitric oxide in tumors: high nitric oxide-adapted head and neck squamous cell carcinoma cell lines
| Autor: | Benjamin J. Vesper, Yaroslav R. Yarmolyuk, Gabor Tarjan, G. Kenneth Haines, Kim M. Elseth, James A. Radosevich, William A. Paradise |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Survival Cell Model system medicine.disease_cause Nitric Oxide Models Biological No donors Nitric oxide chemistry.chemical_compound medicine Tumor Cells Cultured Humans Nitric Oxide Donors Hydrogen peroxide Cell Proliferation General Medicine Hydrogen Peroxide medicine.disease Oxidants Head and neck squamous-cell carcinoma Adaptation Physiological medicine.anatomical_structure chemistry Cell culture Head and Neck Neoplasms Cancer research Carcinoma Squamous Cell Carcinogenesis Nitroso Compounds |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 32(1) |
| ISSN: | 1423-0380 |
| Popis: | The free radical nitric oxide (NO) is over-expressed in many tumors, including head and neck squamous cell carcinomas (HNSCC); however, the role NO plays in tumor pathophysiology is still not well understood. We, herein, report the development of an in vitro model system which can be used to probe the role of NO in the carcinogenesis of HNSCC. Five HNSCC cell lines were adapted to a high NO (HNO) environment by gradually introducing increasing concentrations of DETA-NONOate, a nitrogen-based NO donor, to cell media. The adaptation process was carried out until a sufficiently high enough donor concentration was reached which enabled the HNO cells to survive and grow, but which was lethal to the original, unadapted (“parent”) cells. The adapted HNO cells exhibited analogous morphology to the parent cells, but grew better than their corresponding parent cells in normal media, on soft agar, and in the presence of hydrogen peroxide, an oxygen-based free radical donor. These results indicate that the HNO cell lines are unique and possess biologically different properties than the parent cell lines from which they originated. The HNO/parent cell lines developed herein may be used as a model system to better understand the role NO plays in HNSCC carcinogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink