Cell Apoptosis Control Using BMP4 and Noggin Embedded in a Polyelectrolyte Multilayer Film

Autor: Youssef Haikel, Nadia Benkirane-Jessel, Hajare Mjahed, Bing Hu, Sabine Kuchler-Bopp, Jean-Claude Voegel, Amal Nadiri, Pierre Schaaf
Přispěvatelé: Biomatériaux : Processus biophysiques et biologiques aux interfaces, Université Louis Pasteur - Strasbourg I-Faculté de Chirurgie Dentaire-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de chirurgie dentaire, Université Louis Pasteur - Strasbourg I, Institut Charles Sadron (ICS), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Bouthier, Christiane, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2007
Předmět:
Polymers
MESH: Bone Morphogenetic Proteins
Cell
Apoptosis
Bone Morphogenetic Protein 4
02 engineering and technology
01 natural sciences
MESH: Drug Implants
Electrolytes
chemistry.chemical_compound
MESH: Coated Materials
Biocompatible
MESH: Electrolytes
Coated Materials
Biocompatible
Polylactic Acid-Polyglycolic Acid Copolymer
Polylysine
General Materials Science
Cells
Cultured
Drug Implants
021001 nanoscience & nanotechnology
Polyelectrolyte
MESH: Polymers
Cell biology
Drug Combinations
PLGA
medicine.anatomical_structure
Bone Morphogenetic Proteins
embryonic structures
MESH: Molar
MESH: Membranes
Artificial
0210 nano-technology
MESH: Cells
Cultured
Biotechnology
Programmed cell death
animal structures
Materials science
MESH: Carrier Proteins
010402 general chemistry
Bone morphogenetic protein
Biomaterials
MESH: Polyglycolic Acid
medicine
Humans
Lactic Acid
Noggin
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
MESH: Drug Combinations
MESH: Humans
MESH: Apoptosis
Membranes
Artificial
General Chemistry
Molar
MESH: Polylysine
0104 chemical sciences
[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials
chemistry
Immunology
MESH: Lactic Acid
Bmp antagonist
Carrier Proteins
Polyglycolic Acid
Zdroj: Small
Small, Wiley-VCH Verlag, 2007, 3 (9), pp.1577-83. ⟨10.1002/smll.200700115⟩
Small, 2007, 3 (9), pp.1577-83. ⟨10.1002/smll.200700115⟩
ISSN: 1613-6829
1613-6810
DOI: 10.1002/smll.200700115
Popis: International audience; Programmed cell death (apoptosis) is a genetically regulated process of cell elimination essential during development. During development, programmed cell death is involved in the specific shaping of organs, in the elimination of cells having achieved their program, and in regulating the number of cells to differentiate. Tooth development includes these three aspects and was used here as a model to study the control of apoptosis. Bone morphogenetic proteins (BMPs) are currently considered as playing a major role in signaling apoptosis. This apoptosis could be stopped by treatments with a BMP antagonist ("Noggin"). We selected a model system made by a layer-by-layer approach using poly-L-glutamic acid (PlGA) and poly-L-lysine (PlL) films into which BMP4 and/or Noggin have been embedded. Our results indicate that in situ control of apoptosis during tooth differentiation mediated by both BMP4 and Noggin embedded in a polyelectrolyte multilayer film is possible. We show here for the first time that in the presence of BMP4 and Noggin embedded in a multilayered film, we can induce or inhibit cell death in tooth differentiation, and conserve their biological effects.
Databáze: OpenAIRE
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