Propofol and N-Acetylcysteine attenuate oxidative stress induced by intestinal ischemia/reperfusion in rats: protein carbonyl detection by immunoblotting
| Autor: | Marcelo Aragão Insuellas de Azeredo, Luciana A. I. de Azeredo, Elis C. A. Eleutherio, Alberto Schanaider |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Antioxidant Protein Carbonylation medicine.medical_treatment Ischemia Pharmacology medicine.disease_cause Acetylcysteine Xylazine medicine Animals Ketamine Rats Wistar Propofol Chemistry Free Radical Scavengers medicine.disease Rats Intestines Oxidative Stress Anesthesia Reperfusion Injury Surgery Oxidative stress Biomarkers medicine.drug |
| Zdroj: | Acta cirurgica brasileira. 23(5) |
| ISSN: | 1678-2674 |
| Popis: | PURPOSE: To evaluate the antioxidant effect of Propofol and N-Acetylcysteine (NAC) on intestinal ischemia/reperfusion (I/R) in rats by determining carbonyl protein level. METHODS: Forty Wistar rats were randomly assigned into the following groups: Control; Sham; I/R with Propofol; I/R with Propofol and NAC; I/R with Ketamine and Xylazine. The I/R groups underwent 60 minutes of ischemia and an equal period of reperfusion. Blood samples, collected by cardiac punction, were centrifuged for plasma obtainment. Protein carbonyl level in plasma samples was determined by immunoblotting. RESULTS: No significant difference in protein carbonyl level was found between Control and Sham groups (P>0.05). The highest reduction in protein carbonyl level (P |
| Databáze: | OpenAIRE |
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