Functional changes in murine mammary cancer cells elicited by CoCl2-induced hypoxia
| Autor: | Silvia Vanzulli, Ximena Borenstein, Gabriel L. Fiszman, Maria A. Jasnis, Ada Gabriela Blidner |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Cell Survival Physiology Clinical Biochemistry Biology Nitric Oxide Biochemistry Mice Structure-Activity Relationship Cell Movement In vivo Tumor Cells Cultured medicine Animals Hypoxia Cell Proliferation Mice Inbred BALB C Mammary tumor Dose-Response Relationship Drug Tumor hypoxia Tumor Necrosis Factor-alpha Macrophages Cobalt Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit In vitro Dose–response relationship Cell culture Cancer cell Cancer research medicine.symptom |
| Zdroj: | Nitric Oxide. 23:234-241 |
| ISSN: | 1089-8603 |
| DOI: | 10.1016/j.niox.2010.07.003 |
| Popis: | Low O(2) levels in solid tumors are associated with increase in hypoxia-inducible factor 1alpha (HIF-1alpha). The present study examines functional changes involved in adaptation to hypoxia of the LMM3 mammary tumor cell line, using CoCl(2) as hypoxic mimetic. Our results showed that LMM3 cells were not only tolerant to 150 microM CoCl(2) but they can overgrowth in vitro respect to untreated cells. Hypoxia inhibited cell invasion, migration, MMP-9 activity and NO levels. Macrophage cytotoxicity augmented under hypoxia but was blunted by conditioned media from tumor cells. In vivo tumorigenicity of CoCl(2)-treated cells was greater than controls. Our results show stabilization of HIF-1alpha in LMM3 cells under CoCl(2) and functional changes associated with enhanced cell survival and growth but not with tumor dissemination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect