Exogenous p53 Upregulated Modulator of Apoptosis (PUMA) Decreases Growth of Lung Cancer A549 Cells
| Autor: | Jun-Ping Yang, Xiao-Jun Yang, Wei-Feng Zhu, Xiali Zhang, Huifang Wan, Da-Ya Luo, Chun-Ju Liu, Fusheng Wan |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Lung Neoplasms endocrine system diseases Epidemiology Blotting Western Mice Nude Antineoplastic Agents Apoptosis Flow cytometry Immunoenzyme Techniques Mice Bcl-2-associated X protein Carcinoma Non-Small-Cell Lung Proto-Oncogene Proteins hemic and lymphatic diseases Puma Tumor Cells Cultured medicine Animals Humans p53 upregulated modulator of apoptosis Cell Proliferation bcl-2-Associated X Protein A549 cell Cisplatin Mice Inbred BALB C medicine.diagnostic_test biology Cell growth Public Health Environmental and Occupational Health Flow Cytometry biology.organism_classification Xenograft Model Antitumor Assays Molecular biology Proto-Oncogene Proteins c-bcl-2 Oncology Drug Resistance Neoplasm biology.protein biological phenomena cell phenomena and immunity Apoptosis Regulatory Proteins medicine.drug |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 16:741-746 |
| ISSN: | 1513-7368 |
| DOI: | 10.7314/apjcp.2015.16.2.741 |
| Popis: | PURPOSE To investigate the influence of exogenous p53 upregulated modulator of apoptosis (PUMA) expression on cell proliferation and apoptosis in human non-small cell lung cancer A549 cells and transplanted tumor cell growth in nude mice. MATERIALS AND METHODS A549 cells were divided into the following groups: control, non- carrier (NC), PUMA (transfected with pCEP4- (HA) 2-PUMA plasmid), DDP (10 μg/mL cisplatin treatment) and PUMA+DDP (transfected with pCEP4-(HA)2-PUMA plasmid and 10 μg/mL cisplatin treatment). The MTT method was used to detect the cell survival rate. Cell apoptosis rates were measured by flow cytometry, and PUMA, Bax and Bcl-2 protein expression levels were measured by Western blotting. RESULTS Compared to the control group, the PUMA, DDP and PUMA+DDP groups all had significantly decreased A549 cell proliferation (p |
| Databáze: | OpenAIRE |
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