Vitrification and transfer of cynomolgus monkey (Macaca fascicularis) embryos fertilized by intracytoplasmic sperm injection
| Autor: | Ryuzo Torii, Hideaki Tsuchiya, J. Yamasaki, Tadashi Sankai, Chizuru Iwatani, J. N. Okahara |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_treatment Biology Two stages Intracytoplasmic sperm injection Cryopreservation Embryo Culture Techniques Andrology Food Animals medicine Animals Vitrification Sperm Injections Intracytoplasmic Blastocyst Small Animals Pregnancy Equine Embryo Embryo Transfer medicine.disease Macaca fascicularis medicine.anatomical_structure embryonic structures Female Animal Science and Zoology |
| Zdroj: | Theriogenology. 76:33-38 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2011.01.010 |
| Popis: | Protocols for cryopreservation of monkey embryos are not well established. The objective of the current study was to determine the efficacy of the polypropylene strip method for cryopreserving cynomolgus monkey embryos. Cynomolgus monkey embryos, 63 and 56 at the 4- to 8-cell and 56 blastocyst stages, respectively, were produced by intracytoplasmic sperm injection and in vitro culture, and vitrified using a polypropylene strip. For these two stages, 95 and 86% survived after thawing and pregnancy rates were 29.2% (7 pregnant females/24 recipients, with three live births) and 0% (n = 16 recipients). These were apparently the first live births obtained from embryos fertilized by ICSI. In conclusion, 4- to 8-cell preimplantation cynomolgus monkey embryos were successfully cryopreserved using a polypropylene strip. |
| Databáze: | OpenAIRE |
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