Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study
| Autor: | Maryam Soleimaninejad, Reza Eftekhar, Khadije Zahabi, Majid Tavafi, Maryam Jafari, Peyman Khosravi, Mohamad Reza Moradkhani, Gholamreza Shahsavari, Shahrokh Bagheri, Reza Mohammadrezaei Kkorramabadi, Sanaz Moghadam, Hassan Ahmadvand |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Renal function 03 medical and health sciences chemistry.chemical_compound Diabetes mellitus Glomerulosclerosis 0302 clinical medicine Oleuropein Alloxan Internal medicine medicine Creatinine Kidney biology business.industry Nitric oxide Myeloperoxidase activity medicine.disease Surgery Kidney function test 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Nephrology 030220 oncology & carcinogenesis Myeloperoxidase biology.protein Rat Original Article business |
| Zdroj: | Journal of Nephropathology |
| ISSN: | 2251-8819 2251-8363 |
| DOI: | 10.15171/jnp.2017.34 |
| Popis: | Background: Oleuropein is a potent antioxidant and free-radical scavenger with antiinflammatory properties. Objectives: In the present study, we evaluated the protective effects of oleuropein on myeloperoxidase (MPO) activity, nitrite, urea, creatinine and glomerulosclerosis in alloxan-induced type 1 diabetic rats. Materials and Methods: Thirty Sprague-Dawley male rats were randomly divided into 3 groups: group 1 as control; group 2 as untreated diabetic; and group 3 as treated with oleuropein 15 mg/kg i.p daily. Diabetes was induced in the second and third groups by subcutaneous alloxan injection. After 48 days, the animals were anaesthetized and then the livers and kidneys were removed immediately and used fresh or kept frozen until MPO activity analysis. Blood samples were also collected before sacrificing to measure nitrite, urea, and creatinine. Kidney paraffin sections were prepared to estimate glomerular volume, leukocyte infiltration, and glomerulosclerosis. Results: Oleuropein significantly decreased leukocyte infiltration and glomerulosclerosis in the treated group compared with the diabetic untreated group. Oleuropein significantly decreased the levels of urea, nitrite, and creatinine in the treated group compared with the diabetic untreated group. Moreover, oleuropein significantly decreased MPO activity in the treated group compared with the diabetic untreated group. Conclusions: Oleuropein has antioxidative and antiatherogenic activities and exerts beneficial effects on inflammation and kidney function test and decreases diabetic complication in diabetic rats. |
| Databáze: | OpenAIRE |
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