Global spread of mouse-adapted Staphylococcus aureus lineages CC1, CC15, and CC88 among mouse breeding facilities
| Autor: | Barbara M. Bröker, Werner Nicklas, Dorothee Grumann, Daniel Schulz, Petra Kirsch, Sarah Johnson, Siouxsie Wiles, Daniel M. Mrochen, Kathleen R. Pritchett-Corning, Janine Gumz, Sabine Berg, Jens van den Brandt, Karine Martelet, Patricia Trübe, Silva Holtfreter |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Methicillin-Resistant Staphylococcus aureus Canada China Staphylococcus aureus Genotype Virulence Factors Lineage (evolution) 030106 microbiology Virulence Biology Breeding medicine.disease_cause Microbiology 03 medical and health sciences Mice Animals Laboratory Germany Drug Resistance Bacterial medicine Animals Colonization Genotyping Immune Evasion General Medicine Staphylococcal Infections Virology Adaptation Physiological United States Anti-Bacterial Agents 030104 developmental biology Infectious Diseases Host adaptation France Mobile genetic elements Multilocus Sequence Typing New Zealand |
| Zdroj: | International journal of medical microbiology : IJMM. 308(6) |
| ISSN: | 1618-0607 |
| Popis: | We previously reported that laboratory mice from all global vendors are frequently colonized with Staphylococcus aureus (S. aureus). Genotyping of a snap sample of murine S. aureus isolates from Charles River, US, showed that mice were predominantly colonized with methicillin-sensitive CC88 strains. Here, we expanded our view and investigated whether laboratory mice from other global animal facilities are colonized with similar strains or novel S. aureus lineages, and whether the murine S. aureus isolates show features of host adaptation. In total, we genotyped 230 S. aureus isolates from various vendor facilities of laboratory mice around the globe (Charles River facilities in the USA, Canada, France, and Germany; another US facility) and university- or company-associated breeding facilities in Germany, China and New Zealand. Spa typing was performed to analyse the clonal relationship of the isolates. Moreover, multiplex PCRs were performed for human-specific virulence factors, the immune-evasion cluster (IEC) and superantigen genes (SAg). We found a total of 58 different spa types that clustered into 15 clonal complexes (CCs). Three of these S. aureus lineages had spread globally among laboratory mice and accounted for three quarters of the isolates: CC1 (13.5%), CC15 (14.3%), and CC88 (47.0%). Compared to human colonizing isolates of the same lineages, the murine isolates frequently lacked IEC genes and SAg genes on mobile genetic elements, implying long-term adaptation to the murine host. In conclusion, laboratory mice from various vendors are colonized with host-adapted S. aureus-strains of a few lineages, predominantly the CC88 lineage. S. aureus researchers must be cautioned that S. aureus colonization might be a relevant confounder in infection and vaccination studies and are therefore advised to screen their mice before experimentation. |
| Databáze: | OpenAIRE |
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