Comparison among bone marrow mesenchymal stem and mononuclear cells to promote functional recovery after spinal cord injury in rabbits
| Autor: | Bárbara de Paula Coelho, Raphael Mansur Medina, Antônio Filipe Braga da Fonseca, Jussara Peters Scheffer, André Lacerda de Abreu Oliveira, Arthur Giraldi-Guimarães |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell type Time Factors RD1-811 H&E stain Bone Marrow Cells Mesenchymal Stem Cell Transplantation Peripheral blood mononuclear cell Transplantation Autologous 03 medical and health sciences 0302 clinical medicine Neural Pathways medicine Animals Transplantation Homologous Rabitts Spinal cord injury Spinal Cord Injuries Bone Marrow Transplantation business.industry Mesenchymal stem cell Recovery of Function Functional recovery medicine.disease Transplantation Disease Models Animal 030104 developmental biology medicine.anatomical_structure Anesthesia Leukocytes Mononuclear Female Surgery Rabbits Bone marrow Tomography X-Ray Computed business 030217 neurology & neurosurgery |
| Zdroj: | Acta Cirúrgica Brasileira v.32 n.12 2017 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Vol 32, Iss 12, Pp 1026-1035 (2017) Acta Cirurgica Brasileira, Volume: 32, Issue: 12, Pages: 1026-1035, Published: DEC 2017 |
| Popis: | Purpose: To investigate the efficacy of allogeneic mesenchymal stem-cells and autologous mononuclear cells to promote sensorimotor recovery and tissue rescue. Methods: Female rabbits were submitted to the epidural balloon inflation method and the intravenous cells administrations were made after 8 hours or seven days after injury induction. Sensorimotor evaluation of the hindlimbs was performed, and the euthanasia was made thirty days after the treatment. Spinal cords were stained with hematoxylin and eosin. Results: Both therapies given 8 hours after the injury promoted the sensorimotor recovery after a week. Only the group treated after a week with mononuclear cells showed no significant recovery at post-injury day 14. In the days 21 and 28, all treatments promoted significant recovery. Histopathological analysis showed no difference among the experimental groups. Our results showed that both bone marrow-derived cell types promoted significant sensorimotor recovery after injury, and the treatment made at least a week after injury is efficient. Conclusion: The possibilities of therapy with bone marrow-derived cells are large, increasing the therapeutic arsenal for the treatment of spinal cord injury. |
| Databáze: | OpenAIRE |
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