MicroRNAs coordinately regulate protein complexes

Autor: Dominik Lutter, Yu Wang, Fabian J. Theis, Klaus F. X. Mayer, Sabine Dietmann, Steffen Sass, Thomas Brabletz, Simone Brabletz, Andreas Ruepp, Ulrike C. Burk, Andreas Kowarsch
Rok vydání: 2011
Předmět:
Cell type
Epithelial-Mesenchymal Transition
Systems biology
Immunoblotting
Biology
DNA-binding protein
03 medical and health sciences
0302 clinical medicine
Structural Biology
Modelling and Simulation
Cell Line
Tumor
microRNA
Protein biosynthesis
Humans
Protein Interaction Maps
lcsh:QH301-705.5
Molecular Biology
030304 developmental biology
Regulation of gene expression
0303 health sciences
Messenger RNA
Applied Mathematics
Computer Science Applications
Cell biology
DNA-Binding Proteins
Alcohol Oxidoreductases
MicroRNAs
lcsh:Biology (General)
Gene Expression Regulation
Multiprotein Complexes
030220 oncology & carcinogenesis
Modeling and Simulation
mir-200 family
interaction network
mesenchymal transition
repressors zeb1
cancer-cells
drosophila
expression
evolution
cluster
genes
Function (biology)
Research Article
Zdroj: BMC Syst. Biol. 5:136 (2011)
BMC Systems Biology
BMC Systems Biology, Vol 5, Iss 1, p 136 (2011)
ISSN: 1752-0509
Popis: Background In animals, microRNAs (miRNAs) regulate the protein synthesis of their target messenger RNAs (mRNAs) by either translational repression or deadenylation. miRNAs are frequently found to be co-expressed in different tissues and cell types, while some form polycistronic clusters on genomes. Interactions between targets of co-expressed miRNAs (including miRNA clusters) have not yet been systematically investigated. Results Here we integrated information from predicted and experimentally verified miRNA targets to characterize protein complex networks regulated by human miRNAs. We found striking evidence that individual miRNAs or co-expressed miRNAs frequently target several components of protein complexes. We experimentally verified that the miR-141-200c cluster targets different components of the CtBP/ZEB complex, suggesting a potential orchestrated regulation in epithelial to mesenchymal transition. Conclusions Our findings indicate a coordinate posttranscriptional regulation of protein complexes by miRNAs. These provide a sound basis for designing experiments to study miRNA function at a systems level.
Databáze: OpenAIRE
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