Direct Measurement of Uptake of Sodium at the Outer Surface of the Frog Skin
| Autor: | Thomas U. L. Biber, Peter F. Curran |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Physiology
Skin Absorption Sodium Inorganic chemistry chemistry.chemical_element In Vitro Techniques Lithium Article chemistry.chemical_compound Non-competitive inhibition Electricity Skin Physiological Phenomena Animals Skin Biological Transport Isotopes of sodium Electrophysiology Microelectrode chemistry Lithium chloride Sodium Isotopes Anura Frog Skin Choline chloride |
| Zdroj: | The Journal of General Physiology |
| ISSN: | 1540-7748 0022-1295 |
| DOI: | 10.1085/jgp.56.1.83 |
| Popis: | A combination of the methods described by Schultz et al. (6) and by Ussing and Zerahn (9) was used to measure directly the unidirectional uptake of sodium from the outside solution into the frog skin, under short-circuit conditions. The sodium uptake was determined at six sodium concentrations ranging from 3.4 to 114 mM. NaCl was replaced by choline chloride in the solutions bathing both sides of the skin. Sodium uptake is not a linear function of sodium concentration but appears to be composed of two components, a saturating one and one that varies linearly with concentration. The sodium uptake is inhibited by the addition of lithium to the outside solution. The effect appears to be primarily on the saturating component and has the characteristics of competitive inhibition. In addition, lithium uptake by the skin is inhibited by sodium. The effects of lithium cannot be ascribed to changes in electrical potential difference. Measurements with microelectrodes indicate that under short-circuit condition there is no change in the intracellular potential when lithium chloride is added to the outside solution. |
| Databáze: | OpenAIRE |
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