Generation of cell hybrids via a fusogenic cell line
| Autor: | Thierry Velu, Richard G. Vile, Anne-Nicole Brandenburger, Siew Chiat Cheong, Catherine Bruyns, Jean-Denis Franssen, Myrto Trakatelli, Vy A. Phan, Isabelle Blangenois, Charlotte Servais |
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| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_treatment
Antigen presentation Gene Expression CHO Cells Hybrid Cells Cancer Vaccines Electrofusion Cell Fusion Antigen Transduction Genetic Cell Line Tumor Cricetinae Drug Discovery Genetics medicine Cytotoxic T cell Animals Humans Molecular Biology Genetics (clinical) Fusion biology Immunotherapy Dendritic Cells Molecular biology Coculture Techniques Cell biology Membrane glycoproteins Cell culture biology.protein Molecular Medicine Viral Fusion Proteins HeLa Cells |
| Zdroj: | The journal of gene medicine. 8(7) |
| ISSN: | 1099-498X |
| Popis: | Background Hybrids obtained by fusion between tumour cells (TC) and dendritic cells (DC) have been proposed as anti-tumour vaccines because of their potential to combine the expression of tumour-associated antigens with efficient antigen presentation. The classical methods used for fusion, polyethylene glycol (PEG) and electrofusion, are cytotoxic and generate cell debris that can be taken up by DC rendering the identification of true hybrids difficult. Methods We have established a stable cell line expressing a viral fusogenic membrane glycoprotein (FMG) that is not itself susceptible to fusion. This cell line has been used to generate hybrids and to evaluate the relevance of tools used for hybrid detection. Results This FMG-expressing cell line promotes fusion between autologous or allogeneic TC and DC in any combination, generating ‘tri-parental hybrids’. At least 20% of TC are found to be integrated into hybrids. Conclusions It is speculated that this tri-parental hybrid approach offers new possibilities to further modulate the anti-tumour effect of the DC/TC hybrids since it allows the expression of relevant immunostimulatory molecules by appropriate engineering of the fusogenic cell line. Copyright © 2006 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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