A closer look to cell structural barriers affecting starch digestibility in beans
| Autor: | Vincenzo Fogliano, Edoardo Capuano, Ana M. Rovalino-Córdova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Polymers and Plastics Starch In-vitro starch digestion Cell Sus scrofa Bioaccessibility Cell wall 03 medical and health sciences chemistry.chemical_compound Hydrolysis 0404 agricultural biotechnology Cell Wall Materials Chemistry medicine Animals Amylase Microstructure VLAG chemistry.chemical_classification 030109 nutrition & dietetics biology Organic Chemistry Monosaccharides food and beverages Fabaceae 04 agricultural and veterinary sciences 040401 food science Cytosol Kinetics medicine.anatomical_structure Enzyme Food Quality and Design chemistry Biochemistry Kidney beans biology.protein Biophysics Digestion Cotyledon Cell walls |
| Zdroj: | Carbohydrate Polymers, 181, 994-1002 Carbohydrate Polymers 181 (2018) |
| ISSN: | 0144-8617 |
| Popis: | Isolated bean cells were used to understand the contribution of cell wall and cytoplasmic matrix on starch digestibility. Cotyledon cells were treated enzymatically and mechanically to reduce the level of cell intactness. SEM and chemical characterization revealed that enzymatic treatment modified cell wall thickness and porosity without altering the cytoplasmic matrix, whereas mechanical treatment completely disrupted cell structure. Decreasing cell intactness increased the rate but not the extent of starch digestion in-vitro. It was concluded that cell wall serves as a permeable barrier limiting the access of digestive enzymes. Cytoplasmic matrix, on the other hand, reduced further the accessibility of amylase to starch affecting its hydrolysis rate. In addition, it was proven that cell structural changes, if any, occurring during digestion had no effect on starch hydrolysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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