Cloning of mouse ojoplano, a reticular cytoplasmic protein expressed during embryonic development
| Autor: | Tobias Nolden, Hans Lehrach, Heinz Himmelbauer, Joachim Wittbrodt, Florian Mertes, Ralf Spörle, Juan Ramón Martínez-Morales |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Neural retina epithelium
Neural crest cells Molecular Sequence Data Oryzias Embryonic Development In situ hybridization Biology Mouse embryogenesis Homology (biology) Mice Morphogenesis Genetics Animals Humans OFC1 Amino Acid Sequence Cloning Molecular Eye Proteins Molecular Biology Endoplasmic reticulum Embryogenesis Cleft lip with or without cleft palate Proteins Neural crest Skeletal expression Orofacial cleft 1 Anatomy Embryo Mammalian Fusion protein Cell biology Eye anglage Placodal structures Neural Crest Cytoplasm Ojoplano (OPO) Reticular connective tissue Female Sequence Alignment Developmental Biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 6 páginas, 4 figuras, 1 tabla de sequencia de datos (NCBI accession number EU683438). Ojoplano (Opo) is a morphogenetic gene playing an important role during embryogenesis in medaka. This report focuses on the identification and characterization of the mouse Opo gene. We examined Opo expression by whole-mount in situ hybridization and in situ hybridization on sagittal sections during mouse embryogenesis. First expression in whole-mounts was detected at Theiler stages 15–17 (E 9.5–10.5 dpc) as a spotted specific staining in migrating neural crest cells and in placodal structures. A complex expression pattern was observed in Theiler stage 22–23 (E 14.5 dpc) in sagittal sections, including expression in skeletal structures (skull, vertebrae, ribs, bones of the locomotor system), in the nasal region, the heart and the eye. Fusion proteins revealed the localization of OPO within the cytoplasm with a reticular distribution that largely overlapped with the endoplasmic reticulum. Opo shows homology to human transcripts linked to a hereditary craniofacial malformation, orofacial cleft 1 (OFC1). The expression of mouse Opo in neural crest derivatives and skull elements further supports this link. This work was supported by the Max Planck Society. |
| Databáze: | OpenAIRE |
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