Immunoperoxidase Localization of Apolipoprotein D in Human Enterocytes and Hepatocytes
| Autor: | M Ayrault-Jarrier, N Verthier, M.E. Bouma, A Raisonnier, J. P. de Bandt, J Burdin |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Adult
Gene isoform Antiserum Apolipoprotein D Apolipoprotein B biology Immunoperoxidase Liver cytology Immune Sera Gastroenterology Intestinal epithelium Immunoenzyme Techniques Apolipoproteins Liver Biochemistry Cytoplasm biology.protein Humans lipids (amino acids peptides and proteins) Intestinal Mucosa Apolipoproteins D |
| Zdroj: | Scandinavian Journal of Gastroenterology. 23:477-483 |
| ISSN: | 1502-7708 0036-5521 |
| DOI: | 10.3109/00365528809093897 |
| Popis: | In this study we prepared a pure apolipoprotein D and obtained a specific antiserum to it. The purified apolipoprotein D migrated as a single band of Mr = 29,000 but appeared as five isoforms on isoelectrofocusing. The antiserum did not cross-react with other apolipoproteins. Immunoenzymatic staining revealed the presence of apolipoprotein D in the perinuclear area of the cytoplasm of isolated normal hepatocytes and HepG2 cells. Apolipoprotein D was also localized in intestinal epithelium and in liver cells. The intracellular distribution of apolipoprotein D was similar to that of apolipoprotein B. Our results indicated that apolipoprotein D, like many other circulating apolipoproteins, is synthesized in enterocytes and hepatocytes. |
| Databáze: | OpenAIRE |
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