Lipoic acid reduces glycemia and increases muscle GLUT4 content in streptozotocin-diabetic rats
| Autor: | Mogher Khamaisi, Klaus Wessel, Nava Bashan, Amir Tirosh, Eran Demshchak, Hans Tritschler, Assaf Rudich, Ruth Potashnik |
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| Rok vydání: | 1997 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Monosaccharide Transport Proteins Endocrinology Diabetes and Metabolism medicine.medical_treatment Glucose uptake Muscle Proteins Biology Deoxyglucose In Vitro Techniques Diabetes Mellitus Experimental Rats Sprague-Dawley Gastrocnemius muscle chemistry.chemical_compound Endocrinology Internal medicine medicine Glucose homeostasis Animals Insulin Lactic Acid RNA Messenger Muscle Skeletal Soleus muscle Glucose Transporter Type 4 Thioctic Acid Skeletal muscle Rats Lipoic acid medicine.anatomical_structure chemistry biology.protein GLUT4 |
| Zdroj: | Metabolism: clinical and experimental. 46(7) |
| ISSN: | 0026-0495 |
| Popis: | Alpha lipoic acid (lipoate [LA]), a cofactor of alpha-ketodehydrogenase, exhibits unique antioxidant properties. Recent studies suggest a direct effect of LA on glucose metabolism in both human and experimental diabetes. This study examines the possibility that LA positively affects glucose homeostasis in streptozotocin (STZ)-induced diabetic rats by altering skeletal muscle glucose utilization. Blood glucose concentration in STZ-diabetic rats following 10 days of intraperitoneal (i.p.) injection of LA 30 mg/kg was reduced compared with that in vehicle-treated diabetic rats (495 +/- 131 v 641 +/- 125 mg/dL in fed state, P = .003, and 189 +/- 48 v 341 +/- 36 mg/dL after 12-hour fast, P = .001). No effect of LA on plasma insulin was observed. Gastrocnemius muscle crude membrane GLUT4 protein was elevated both in control and in diabetic rats treated with LA by 1.5- and 2.8-fold, respectively, without significant changes in GLUT4 mRNA levels. Gastrocnemius lactic acid was increased in diabetic rats (19.9 +/- 5.5 v 10.4 +/- 2.8 mumol/g muscle, P < .05 v nondiabetic rats), and was normal in LA-treated diabetic rats (9.1 +/- 5.0 mumol/g muscle). Insulin-stimulated 2-deoxyglucose (2 DG) uptake into isolated soleus muscle was reduced in diabetic rats compared with the control group (474 +/- 15 v 568 +/- 52 pmol/mg muscle 30 min, respectively, P = .05). LA treatment prevented this reduction, resulting in insulin-stimulated glucose uptake comparable to that of nondiabetic animals. These results suggest that daily LA treatment may reduce blood glucose concentrations in STZ-diabetic rats by enhancing muscle GLUT4 protein content and by increasing muscle glucose utilization. |
| Databáze: | OpenAIRE |
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