Modulation by topiramate of AMPA and kainate mediated calcium influx in cultured cerebral cortical, hippocampal and cerebellar neurons
| Autor: | Timothy A. Simeone, H.Steven White, Arne Schousboe, Thomas E. Maar, Virginia Smith-Swintosky, Claus F. Poulsen |
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| Rok vydání: | 2004 |
| Předmět: |
Topiramate
Cerebellum Hippocampus Kainate receptor AMPA receptor Fructose Pharmacology Biochemistry Cellular and Molecular Neuroscience Mice Pregnancy medicine Animals alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Cerebral Cortex Neurons Ion Transport Kainic Acid Chemistry Glutamate receptor General Medicine medicine.anatomical_structure nervous system Cerebral cortex NMDA receptor Calcium Female medicine.drug |
| Zdroj: | Neurochemical research. 29(1) |
| ISSN: | 0364-3190 |
| Popis: | The effect of the antiepileptic drug topiramate on Ca2+ uptake through (RS)-2-amino-3-(3-hydroxy-5-methylisoxazol-4-yl)propionate (AMPA) and kainate (KA) receptors was investigated in different cell culture systems consisting of neurons from the cerebral cortex, hippocampus, and cerebellum. Ca2+ influx was assayed using a fluorescent Ca2+ chelator to monitor changes in the intracellular Ca2+ concentration or cobalt staining to assess the effect of topiramate on Ca2+-permeable AMPA/KA receptors. In all types of neuronal cultures studied, AMPA and KA were found to elicit an influx of Ca2+ in a subset of the neuronal population. Topiramate, at concentrations of 30 and 100 microM, inhibited Ca2+ influx by up to 60%. Modulation of AMPA and KA-evoked Ca2+ influx may contribute to both the antiepileptic and neuroprotective properties of topiramate. |
| Databáze: | OpenAIRE |
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