Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease
| Autor: | Santanu Dasgupta, Julie V. Philley, Varsha Taskar, David E. Griffith, James G. Fox, Megan S. Devine, Foram Thakkar, Anbarasu Kannan, Hernaina Bains, Jeana L. Benwill, Richard J. Wallace, Barbara A. Brown-Elliott, Ammar Alqaid, Sudeep Gupta |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Epithelial-Mesenchymal Transition T-Lymphocytes medicine.medical_treatment Blotting Western Fluorescent Antibody Technique Breast Neoplasms Exosomes Exosome Mass Spectrometry nontuberculous Mycobacterium 03 medical and health sciences Extracellular matrix protein 1 breast cancer 0302 clinical medicine Breast cancer medicine Humans ECM1 Epithelial–mesenchymal transition skin and connective tissue diseases education Aged Aged 80 and over Extracellular Matrix Proteins Mycobacterium Infections education.field_of_study business.industry AZGP1 Middle Aged Flow Cytometry medicine.disease Coculture Techniques Microvesicles Bronchiectasis 030104 developmental biology Cytokine Oncology 030220 oncology & carcinogenesis Immunology biomarker Biomarker (medicine) Female Transcriptome business Signal Transduction Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.14964 |
| Popis: | // Julie V. Philley 1 , Anbarasu Kannan 2 , David E. Griffith 1 , Megan S. Devine 1 , Jeana L. Benwill 1 , Richard J. Wallace, Jr. 1, 3 , Barbara A. Brown-Elliott 3 , Foram Thakkar 1 , Varsha Taskar 1 , James G. Fox 1 , Ammar Alqaid 1 , Hernaina Bains 1 , Sudeep Gupta 4 , Santanu Dasgupta 2 1 Departments of Medicine, The University of Texas Health Science Center at Tyler, Texas, USA 2 Cellular and Molecular Biology, The University of Texas Health Science Center at Tyler, Texas, USA 3 The Mycobacteria/Nocardia Research Laboratory Department of Microbiology, The University of Texas Health Science Center at Tyler, Texas, USA 4 Medical Oncology, Tata Memorial Center, Mumbai, India Correspondence to: Santanu Dasgupta, email: santanu.dasgupta@uthct.edu Keywords: Exosomes, breast cancer, nontuberculous Mycobacterium, ECM1, biomarker Received: July 10, 2016 Accepted: January 10, 2017 Published: February 01, 2017 ABSTRACT Bronchiectasis Nontuberculous mycobacterium (NTM nb ) infection is an emerging health problem in breast cancer (BCa) patients. We measured sera exosome proteome in BCa-NTM nb subjects and controls by Mass Spectroscopy. Extracellular matrix protein 1 (ECM1) was detected exclusively in the circulating exosomes of 82% of the BCa-NTM nb cases. Co-culture of ECM1 + exosomes with normal human mammary epithelial cells induced epithelial to mesenchymal transition accompanied by increased Vimentin/CDH1 expression ratio and Glutamate production. Co-culture of the ECM1 + exosomes with normal human T cells modulated their cytokine production. The ECM1 + exosomes were markedly higher in sera obtained from BCa-NTM nb subjects. Exclusive expression of APN, APOC4 and AZGP1 was evident in the circulating exosomes of these BCa-NTM nb cases, which predicts disease prevalence independent of the body max index in concert with ECM1. Monitoring ECM1, APN, APOC4 and AZGP1 in the circulating exosomes could be beneficial for risk assessment, monitoring and surveillance of BCa-NTM nb . |
| Databáze: | OpenAIRE |
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