Endophyte fungi, Cladosporium species-mediated synthesis of silver nanoparticles possessing in vitro antioxidant, anti-diabetic and anti-Alzheimer activity

Autor:	Ravishankar Vittal Rai, M. Govindappa, V R Ranjitha, Akshatha B. Subramanyam, Vishaka Anil, Dimple Popli, Saroja N. Rao, M N Namratha
Rok vydání:	2018
Předmět:	0301 basic medicine Silver Antioxidant medicine.medical_treatment Biomedical Engineering Metal Nanoparticles Pharmaceutical Science Medicine (miscellaneous) 02 engineering and technology Endophyte Antioxidants Plant use of endophytic fungi in defense Silver nanoparticle Microbiology 03 medical and health sciences Alzheimer Disease medicine Hypoglycemic Agents Nanotechnology Anti alzheimer biology Chemistry General Medicine 021001 nanoscience & nanotechnology biology.organism_classification In vitro Cladosporium species 030104 developmental biology 0210 nano-technology Cladosporium Biotechnology
Zdroj:	Artificial Cells, Nanomedicine, and Biotechnology. 46:676-683
ISSN:	2169-141X 2169-1401
Popis:	This research study is to develop an easy and eco-friendly method for the synthesis of AgNPs using aqueous extract of endophytic fungi, Cladosporium species (CsAgNPs) and investigated the effects of antioxidant, anti-diabetic and anti-acetylcholinesterase (AChE), anti-butyrylcholinesterase (BChE) activity. Cladosporium species-mediated silver nanoparticles were characterized by UV-Vis spectrophotometer, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). The aqueous extract of Cladosporium species has shown the presence of carbohydrates, tannin, phenolic glycosides, terpenoids, alkaloids, phenol and anthraquinones. At 438 nm conformed the absorbance of AgNPs. The SEM result confirms that size, morphology and high density of the synthesized nanoparticles with huge disparity in the particle size distribution. The FTIR analysis confirmed the important biological compounds responsible for reduction of silver. Strong absorption property of AgNPs was studied by EDX. In antioxidant activity, CsAgNPs showed the involvement of NADPH-dependent reductase in the formation of AgNPs. The AgNPs has reduced the activity of α-amylase, α-glucosidase and dipeptidyl peptidase IV in vitro antidiabetic activity. The CsAgNPs showed significant glucose uptake in 3T3L1 cell line. The AgNPs have shown excellent inhibitory activity against AChE and BChE. To our best knowledge, this is the first on the synthesis of silver nanoparticles using endophytic fungi, Cladosporium species isolated from healthy leaf of Loranthus micranthus. Hence, to validate our results the in vivo animal studies at molecular level are needed to develop an antioxidant, anti-diabetic and anti-cholinesterase agent.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9811e0127e83d5d214d68f86b45afe84 https://doi.org/10.1080/21691401.2018.1434188 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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