Antioxidant activity and inhibition of ultraviolet radiation-induced skin damage of Selenium-rich peptide fraction from selenium-rich yeast protein hydrolysate
| Autor: | Siyu Guo, Hongmei Liu, Hengke Guo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
Cell Survival Protein Hydrolysates Ultraviolet Rays medicine.medical_treatment chemistry.chemical_element Administration Oral Fraction (chemistry) Peptide Mice Inbred Strains Saccharomyces cerevisiae Protective Agents 01 natural sciences Biochemistry Hydrolysate Antioxidants Lipid peroxidation chemistry.chemical_compound Mice Selenium Structure-Activity Relationship Picrates Drug Discovery medicine Animals Humans Benzothiazoles Molecular Biology Cells Cultured Skin chemistry.chemical_classification Dose-Response Relationship Drug Molecular Structure 010405 organic chemistry Organic Chemistry Biphenyl Compounds Hydrogen Peroxide Yeast In vitro 0104 chemical sciences 010404 medicinal & biomolecular chemistry Oxidative Stress chemistry Female Sulfonic Acids Peptides |
| Zdroj: | Bioorganic chemistry. 105 |
| ISSN: | 1090-2120 |
| Popis: | The bioactive peptides and trace element selenium (Se) both have good antioxidant activity. However, whether combined Se and bioactive peptides have more excellent antioxidant activity remain unknown. The aim of this study is to prepare a Se-rich peptide fraction containing both Se and peptides from Se-rich yeast protein hydrolysate and investigated its antioxidant activity and effect on ultraviolet B (UVB) radiation-induced skin oxidative damage. The peptide fractions with different molecular weight (MW) and Se content were obtained by enzymatically hydrolyzing normal or Se-rich yeast proteins followed by a filtration process. In vitro free radical scavenging and lipid peroxidation inhibition assays showed that Se-rich peptides fraction with lower MW of 1 kDa (sSeP) had the highest antioxidant activity compared with Se-rich peptide fractions with higher MW of 3 kDa or normal peptide fractions. Oral administration of sSeP significantly decreased the level of malonaldehyde (MDA) in liver and serum, and increased the activity of glutathione peroxidase (GPx) in liver and serum in normal mice. When topically applied on the dorsal skin of mice, sSeP effectively alleviate UVB radiation-induced skin damage and oxidative stress by increasing GPx and catalase activities and glutathione content in skin or serum. Furthermore, sSeP showed a protective effect against H |
| Databáze: | OpenAIRE |
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