Study on the common teratogenic pathway elicited by the fungicides triazole-derivatives
| Autor: | F. Di Renzo, Erminio Giavini, M. L. Broccia, Valentina Massa, Elena Menegola |
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| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
animal structures Morphogenesis Rhombomere Embryonic Development Hindbrain Biology Toxicology Nervous System Malformations Pregnancy Internal medicine Pharyngeal apparatus medicine Animals Early Growth Response Protein 2 Embryogenesis Days post coitum Cranial Nerves Neural crest General Medicine Triazoles Immunohistochemistry Cell biology Fungicides Industrial Rats DNA-Binding Proteins Rhombencephalon Endocrinology medicine.anatomical_structure Teratogens Neural Crest embryonic structures Pharynx Female Pharyngeal arch Biomarkers Transcription Factors |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 19(6) |
| ISSN: | 0887-2333 |
| Popis: | Triazole-derivatives alter the pharyngeal apparatus morphogenesis of rodent embryos cultured in vitro. The hindbrain segmentation and the rhombencephalic neural crest cell (NCCs) migration are altered by Fluconazole exposure in vitro. The aim of the present work is to identify if a common pathogenic pathway is detectable also for other molecules of this class of compounds. 9.5 days post coitum (d.p.c.) old rat embryos were exposed in vitro to the teratogenic concentrations of Flusilazole, Triadimefon and Triadimenol and cultured for 24, 48 or 60 h. The expression and localisation of Hox-b1 and Krox-20 proteins (used as markers for hindbrain segmentation) were evaluated after 24 h of culture. The localisation and distribution of NCC was evaluated after 24, 30 and 48 h of culture. The morphology of the embryos was analysed after 48 h, while the branchial nerve structures were evaluated after 60 h of culture. Hindbrain segmentation and NCC migration alteration as well as pharyngeal arch and cranial nerve abnormalities were detected after exposure of the tested molecules. A common severe teratogenic intrinsic property for the tested molecules of this chemical class has been found, acting through alteration of the normal hindbrain developmental pattern. |
| Databáze: | OpenAIRE |
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