Novel Hairpin-Shaped Primer Assay To Study the Association of the −44 Single-Nucleotide Polymorphism of theDEFB1Gene with Early-Onset Periodontal Disease
| Autor: | Manzour Hernando Hazbón, Joyce Eskdale, Grant Gallagher, Greig P. Lennon, William J. Jordan, Michele Boniotto, David Alland |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
beta-Defensins Genotype Clinical Biochemistry Immunology Single-nucleotide polymorphism Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Sensitivity and Specificity law.invention chemistry.chemical_compound law Ethnicity Humans Immunology and Allergy SNP Allele Transversion Alleles Periodontal Diseases Polymerase chain reaction DNA Primers Genetics Molecular biology chemistry Immune-Mediated Responses and Disorders SYBR Green I Primer (molecular biology) |
| Zdroj: | Clinical and Vaccine Immunology. 11:766-769 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cdli.11.4.766-769.2004 |
| Popis: | A powerful, cost-effective new method for studying single-nucleotide polymorphisms (SNPs) is described. This method is based on the use of hairpin-shaped primers (HP), which give a sensitive and specific PCR amplification of each specific allele, without the use of costly fluorophore-labeled probes and any post-PCR manipulation. The amplification is monitored in real-time using SYBR Green I dye and takes only 2 h to yield results. The HP assay has a simple design and utilizes a conventional real-time PCR apparatus. The −44 C→G transversion in theDEFB1gene (which encodes human β-defensin 1) has been previously associated withCandidacarriage in oral epithelia. In this study, we analyzed the association between early-onset periodontal disease (EOP) and the −44 SNP. We used an HP assay to study the distribution of the −44 SNP in 264 human DNAs obtained from two cohorts of EOP patients and healthy controls from different ethnic backgrounds. The results indicate that the −44 SNP has a similar distribution between EOP and healthy patients, suggesting that it is not associated with the disease. |
| Databáze: | OpenAIRE |
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