Proteomic Landscape Has Revealed Small Rubber Particles Are Crucial Rubber Biosynthetic Machines for Ethylene-Stimulation in Natural Rubber Production

Autor: Xuchu Wang, Li Yu, Pascal Montoro, Zheng Tong, Xueyan Zhang, Yong Sun, Hongbin Li, Quanliang Xie, Lili Chang, Daniel I. C. Wang, Xuelin Jin, He Peng, Yiyang Dong, Boxuan Yuan
Rok vydání: 2019
Předmět:
Proteomics
0106 biological sciences
0301 basic medicine
Proteome
Latex
01 natural sciences
lcsh:Chemistry
J15 - Manutention
transport
stockage et conservation des produits agricoles non-alimentaires
Serine
chemistry.chemical_compound
Protein phosphorylation
Phosphorylation
lcsh:QH301-705.5
Spectroscopy
Plant Proteins
chemistry.chemical_classification
biology
Chemistry
General Medicine
small rubber particle
J11 - Manutention
transport
stockage et conservation des produits d'origine végétale
Computer Science Applications
Amino acid
Hevea brasiliensis
Biochemistry
visual_art
visual_art.visual_art_medium
quantitative proteomics
Quantitative proteomics
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Natural rubber
Biosynthesis
Synthèse protéique
Amino Acid Sequence
Physical and Theoretical Chemistry
Molecular Biology
Caoutchouc
Organic Chemistry
Antigens
Plant
Ethylenes
biology.organism_classification
protein phosphorylation
Elongation factor
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Hevea
Rubber
natural rubber biosynthesis
Éthylène
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 20
International Journal of Molecular Sciences, Vol 20, Iss 20, p 5082 (2019)
ISSN: 1422-0067
Popis: Rubber particles are a specific organelle for natural rubber biosynthesis (NRB) and storage. Ethylene can significantly improve rubber latex production by increasing the generation of small rubber particles (SRPs), regulating protein accumulation, and activating many enzyme activities. We conducted a quantitative proteomics study of different SRPs upon ethylene stimulation by differential in-gel electrophoresis (DIGE) and using isobaric tags for relative and absolute quantification (iTRAQ) methods. In DIGE, 79 differentially accumulated proteins (DAPs) were determined as ethylene responsive proteins. Our results show that the abundance of many NRB-related proteins has been sharply induced upon ethylene stimulation. Among them, 23 proteins were identified as rubber elongation factor (REF) and small rubber particle protein (SRPP) family members, including 16 REF and 7 SRPP isoforms. Then, 138 unique phosphorylated peptides, containing 129 phosphorylated amino acids from the 64 REF/SRPP family members, were identified, and most serine and threonine were phosphorylated. Furthermore, we identified 226 DAPs from more than 2000 SRP proteins by iTRAQ. Integrative analysis revealed that almost all NRB-related proteins can be detected in SRPs, and many proteins are positively responsive to ethylene stimulation. These results indicate that ethylene may stimulate latex production by regulating the accumulation of some key proteins. The phosphorylation modification of REF and SRPP isoforms might be crucial for NRB, and SRP may act as a complex natural rubber biosynthetic machine.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje