Proteomic Landscape Has Revealed Small Rubber Particles Are Crucial Rubber Biosynthetic Machines for Ethylene-Stimulation in Natural Rubber Production
Autor: | Xuchu Wang, Li Yu, Pascal Montoro, Zheng Tong, Xueyan Zhang, Yong Sun, Hongbin Li, Quanliang Xie, Lili Chang, Daniel I. C. Wang, Xuelin Jin, He Peng, Yiyang Dong, Boxuan Yuan |
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Rok vydání: | 2019 |
Předmět: |
Proteomics
0106 biological sciences 0301 basic medicine Proteome Latex 01 natural sciences lcsh:Chemistry J15 - Manutention transport stockage et conservation des produits agricoles non-alimentaires Serine chemistry.chemical_compound Protein phosphorylation Phosphorylation lcsh:QH301-705.5 Spectroscopy Plant Proteins chemistry.chemical_classification biology Chemistry General Medicine small rubber particle J11 - Manutention transport stockage et conservation des produits d'origine végétale Computer Science Applications Amino acid Hevea brasiliensis Biochemistry visual_art visual_art.visual_art_medium quantitative proteomics Quantitative proteomics Article Catalysis Inorganic Chemistry 03 medical and health sciences Natural rubber Biosynthesis Synthèse protéique Amino Acid Sequence Physical and Theoretical Chemistry Molecular Biology Caoutchouc Organic Chemistry Antigens Plant Ethylenes biology.organism_classification protein phosphorylation Elongation factor 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Hevea Rubber natural rubber biosynthesis Éthylène 010606 plant biology & botany |
Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 20 International Journal of Molecular Sciences, Vol 20, Iss 20, p 5082 (2019) |
ISSN: | 1422-0067 |
Popis: | Rubber particles are a specific organelle for natural rubber biosynthesis (NRB) and storage. Ethylene can significantly improve rubber latex production by increasing the generation of small rubber particles (SRPs), regulating protein accumulation, and activating many enzyme activities. We conducted a quantitative proteomics study of different SRPs upon ethylene stimulation by differential in-gel electrophoresis (DIGE) and using isobaric tags for relative and absolute quantification (iTRAQ) methods. In DIGE, 79 differentially accumulated proteins (DAPs) were determined as ethylene responsive proteins. Our results show that the abundance of many NRB-related proteins has been sharply induced upon ethylene stimulation. Among them, 23 proteins were identified as rubber elongation factor (REF) and small rubber particle protein (SRPP) family members, including 16 REF and 7 SRPP isoforms. Then, 138 unique phosphorylated peptides, containing 129 phosphorylated amino acids from the 64 REF/SRPP family members, were identified, and most serine and threonine were phosphorylated. Furthermore, we identified 226 DAPs from more than 2000 SRP proteins by iTRAQ. Integrative analysis revealed that almost all NRB-related proteins can be detected in SRPs, and many proteins are positively responsive to ethylene stimulation. These results indicate that ethylene may stimulate latex production by regulating the accumulation of some key proteins. The phosphorylation modification of REF and SRPP isoforms might be crucial for NRB, and SRP may act as a complex natural rubber biosynthetic machine. |
Databáze: | OpenAIRE |
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