Determination of saponins and alkaloids inCaulophyllum thalictroides (blue cohosh) by high-performance liquid chromatography and evaporative light scattering detection
| Autor: | Markus Ganzera, H. R. W. Dharmaratne, Ikhlas A. Khan, N. P. D. Nanayakkara |
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| Rok vydání: | 2003 |
| Předmět: |
Caulophyllum thalictroides
Plant Science Plant Roots complex mixtures Biochemistry High-performance liquid chromatography Mass Spectrometry Light scattering Analytical Chemistry chemistry.chemical_compound Alkaloids Triterpene Drug Discovery Scattering Radiation Acetonitrile Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Molecular Structure Alkaloid General Medicine Saponins Triterpenes Caulophyllum Complementary and alternative medicine chemistry Stationary phase Molecular Medicine Spectrophotometry Ultraviolet Ammonium acetate Food Science |
| Zdroj: | Phytochemical Analysis. 14:1-7 |
| ISSN: | 1099-1565 0958-0344 |
| DOI: | 10.1002/pca.666 |
| Popis: | The roots of Caulophyllum thalictroides, traditionally used for the treatment of menstrual difficulties and as an aid in childbirth, contain saponins, which are considered to be responsible for the uterine stimulant effects, together with teratogenic alkaloids. An HPLC method has been developed which permits the determination of the triterpene saponins in the plant and also the separation of four alkaloids. The best results were obtained with a C-12 stationary phase using ammonium acetate buffer (pH 8.0) and acetonitrile as mobile phase. Owing to their low UV absorbance, the saponins were detected by evaporative light scattering, whereas the alkaloids were monitored by UV at 310 nm. The identities of the compounds were confirmed in an LC-MS experiment. Different plant samples and commercial products have been analysed using the described method, and remarkable qualitative and quantitative variations were revealed. Comparing the daily uptake of total saponins, a difference of greater than 100-fold was observed within the various products; the alkaloid content on the other hand was more uniform. Copyright © 2003 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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