IgG recognizing 21-24 kDa and 30-33 kDa tachyzoite antigens show maximum avidity maturation during natural and accidental human toxoplasmosis
| Autor: | Alberto Nieto, Julio Battistoni, Margarita Villavedra |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
lcsh:Arctic medicine. Tropical medicine
Time Factors lcsh:RC955-962 RC955-962 Immunoblotting Antibody Affinity chemical and pharmacologic phenomena Antigens Protozoan Enzyme-Linked Immunosorbent Assay Biology Avidity Antigen Arctic medicine. Tropical medicine medicine Animals Humans Diagnostic Acute phase T. gondii General Medicine Serum samples medicine.disease Virology Toxoplasmosis Specific igm Infectious Diseases Immunoglobulin G Immunology Acute Disease Chronic Disease Toxoplasma Follow-Up Studies |
| Zdroj: | Revista do Instituto de Medicina Tropical de São Paulo, Vol 41, Iss 5, Pp 297-303 (1999) Revista do Instituto de Medicina Tropical de São Paulo, Volume: 41, Issue: 5, Pages: 297-303, Published: SEP 1999 |
| ISSN: | 0036-4665 |
| Popis: | We describe the avidity maturation of IgGs in human toxoplasmosis using sequential serum samples from accidental and natural infections. In accidental cases, avidity increased continuously throughout infection while naturally infected patients showed a different profile. Twenty-five percent of sera from chronic patients having specific IgM positive results could be appropriately classified using exclusively the avidity test data. To take advantage of the potentiality of this technique, antigens recognized by IgG showing steeper avidity maturation were identified using immunoblot with KSCN elution. Two clusters of antigens, in the ranges of 21-24 kDa and 30-33 kDa, were identified as the ones that fulfill the aforementioned avidity characteristics. Descrevemos a avidez de maturação de IgGs na toxoplasmose humana usando amostras sequenciais de soro de infecções naturais e acidentais. Em casos acidentais, a avidez aumentou continuamente através da infeção enquanto os pacientes naturalmente infectados mostraram perfil diferente. 25% dos soros de pacientes crônicos que possuiam resultados positivos de IgM específica puderam ser classificados apropriadamente usando exclusivamente os dados do teste de avidez. Para se aproveitar a potencialidade desta técnica, antígenos reconhecidos pela IgG mostrando progressiva avidez de maturação foram identificados usando-se Imunoblot com eluição com KSCN. Dois grupos de antígenos, com limites de 21-24 kDa e 30-33 kDa, foram identificados como aqueles que preenchem as características de avidez acima mencionadas. |
| Databáze: | OpenAIRE |
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