A natural variant of the essential host gene MMS21 restricts the parasitic 2-micron plasmid in Saccharomyces cerevisiae
| Autor: | Janet M. Young, Paula F. Levan, Harmit S. Malik, Michelle Hays |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins DNA repair QTL QH301-705.5 Science Saccharomyces cerevisiae Genes Fungal Quantitative Trait Loci SUMO-1 Protein S. cerevisiae Genomics Quantitative trait locus General Biochemistry Genetics and Molecular Biology Genomic Instability Chromosome segregation 03 medical and health sciences 0302 clinical medicine Plasmid Biology (General) Mitosis genetic conflicts Repetitive Sequences Nucleic Acid Genetics Evolutionary Biology General Immunology and Microbiology biology Smc5/6 complex General Neuroscience Genetic Variation Genetics and Genomics General Medicine biology.organism_classification natural isolates Establishment of sister chromatid cohesion 030104 developmental biology high-copy plasmids Medicine Single-Cell Analysis Smc5/6 030217 neurology & neurosurgery Research Article Plasmids |
| Zdroj: | eLife, Vol 9 (2020) eLife |
| Popis: | Antagonistic coevolution with selfish genetic elements (SGEs) can drive evolution of host resistance. Here, we investigated host suppression of 2-micron (2μ) plasmids, multicopy nuclear parasites that have co-evolved with budding yeasts. We developed SCAMPR (Single-Cell Assay for Measuring Plasmid Retention) to measure copy number heterogeneity and 2μ plasmid loss in live cells. We identified threeS. cerevisiaestrains that lack endogenous 2μ plasmids and reproducibly inhibit mitotic plasmid stability. Focusing on the Y9 ragi strain, we determined that plasmid restriction is heritable and dominant. Using bulk segregant analysis, we identified a high-confidence Quantitative Trait Locus (QTL) with a single variant ofMMS21associated with increased 2μ instability.MMS21encodes a SUMO E3 ligase and an essential component of the Smc5/6 complex, involved in sister chromatid cohesion, chromosome segregation, and DNA repair. Our analyses leverage natural variation to uncover a novel means by which budding yeasts can overcome highly successful genetic parasites. |
| Databáze: | OpenAIRE |
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