Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro
| Autor: | Walter dos Santos Lima, Tiago Antônio de Oliveira Mendes, Rodolfo Silva Moreira Cezar, Fernanda S.O. Santos, Aytube Lucas Coaglio, Cíntia Aparecida de Jesus Pereira, Fernando Luiz Pereira de Oliveira, Laura L.S. Oliveira, Gustavo Conzensa |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fascioliasis Momordica charantia Population Cattle Diseases Albendazole Feces 03 medical and health sciences chemistry.chemical_compound Hepatica parasitic diseases medicine Animals Fasciola hepatica Fasciolosis education Parasite Egg Count Incubation Chromatography High Pressure Liquid Ovum Anthelmintics Flavonoids education.field_of_study Plants Medicinal General Veterinary Traditional medicine biology Momordica Plant Extracts General Medicine 030108 mycology & parasitology biology.organism_classification medicine.disease Plant Leaves Biochemistry chemistry Cattle Female Quercetin Parasitology medicine.drug |
| Zdroj: | Veterinary Parasitology. 228:160-166 |
| ISSN: | 0304-4017 |
| Popis: | Fasciolosis, a parasitic disease caused by the trematode Fasciola hepatica underreported is expanding both in human and animal population, throughout the world. The constant use of synthetic drugs to treat this condition has led to the natural selection of resistant strains of the parasite. Hence, there is a growing focus on the potential anti-helminthic properties of medicinal plants and phytopharmaceuticals. The current study assessed the potential anti-fasciolicide action of Momordica charantia leaf extracts and fractions on the eggs of F. hepatica parasites. The lyophilized crude extract (CE) of M. charantia leaves and its sub-fractions, obtained from liquid-liquid partitioning with organic solvents, were analysed by High Performance Liquid Chromatography (HPLC), suspended in 1% DMSO and used in in vitro tests. Quadruplicates of 50F. hepatica eggs were incubated at 23°C with M. charantia leaf CE in different concentrations. After 12days no larvae were formed in eggs incubated with CE concentrations above 12.5mg/mL. Eggs incubated with CE sub-fractions at concentrations of 1000, 100, 10, 1, 0.1, 0.01μg/mL affected embryonic development, with n-butanol presenting the strongest inhibition of miracidia formation. In contrast, on the 12th day, 90% of the miracidia hatched in the control experiments using 0.03% DMSO whereas embryogenesis was completely abolished with any concentration of albendazole sulphoxide ABZ(SO). Chemical analysis of the CE and sub-fractions revealed a prominent presence of flavonoids. HPLC-MS confirmed Quercetin to be one of the main flavonoids present in the CE and the n-butanol subfraction. This is the first study to analyse the potential anti-fasciolicide action of M. charantia leaf CE and subfractions. |
| Databáze: | OpenAIRE |
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