Expression of USP2-69 in mesangial cellsin vivoandin vitro
| Autor: | Zhonghua Zhao, Duan Ma, Qi Chen, Suxia Wang, Mu-yi Guo, Jianyong Sun, Zhigang Zhang, Huijuan Wu, Ye Liu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Blotting Western Interleukin-1beta Lupus nephritis Pathology and Forensic Medicine Nephropathy Glomerulonephritis Endopeptidases medicine Animals Humans RNA Messenger Cells Cultured Cell Proliferation Analysis of Variance Kidney biology Mesangial cell Reverse Transcriptase Polymerase Chain Reaction Cell growth Glomerulonephritis IGA General Medicine medicine.disease Immunohistochemistry Lupus Nephritis Rats Up-Regulation Proliferating cell nuclear antigen Isoenzymes medicine.anatomical_structure Mesangial Cells biology.protein Ubiquitin Thiolesterase |
| Zdroj: | Pathology International. 60:184-192 |
| ISSN: | 1440-1827 1320-5463 |
| DOI: | 10.1111/j.1440-1827.2010.02496.x |
| Popis: | Ubiquitin-specific protease 2 (USP2) is a member of a family of de-ubiquitinating enzymes. It may play an important role in the regulation of cell growth and differentiation. It is known that expression of the isoform USP2-69 kD is high in kidney tissue, but its role remains unclear. Mesangial cell proliferation is a prominent element of various types of glomerulonephritides. Therefore, whether USP2 plays a role in mesangial cell proliferation during glomerulonephritides is an interesting question to explore. The purpose of the present study was to evaluate USP2-69 expression in needle biopsies of human kidneys and in cultured rat mesangial cells. On immunohistochemistry USP2-69 was upregulated in some mesangial proliferative glomerulonephritides. The proportion of USP2-69 positive area in the glomeruli was 3.90% in normal kidney, 4.96% in minimal change disease, and 4.39% in membranous glomerulonephritides, while it was 14.84% in IgA nephropathy (IgAN) (mesangial proliferative type), 16.18% in lupus nephritis (LN; diffuse proliferative type) and 15.54% in acute proliferative glomerulonephritides (APGN); the difference of the percentages between IgAN, LN (IV subtype) and APGN and normal kidney were statistically significant (P < 0.05). Additionally, the number of proliferating cell nuclear antigen (PCNA)-positive nuclei in the glomeruli was statistically significantly higher in the various glomerulonephritides than in the normal kidney (P < 0.05). Immunohistochemistry showed that the distribution of the USP2(+) area and PCNA(+) nuclei overlapped in the glomeruli. Treatment with interleukin-1beta for 12 h and 24 h, or with anti-thymocyte serum for 6 h and 12 h resulted in elevated USP2-69 mRNA and protein expression in the rat mesangial cells. Also, PCNA expression increased and p27 expression decreased significantly in the treated mesangial cells. These findings suggest that USP2-69 was upregulated in mesangial cells during mesangial proliferative glomerulonephritides in vivo and in vitro, which may relate to the proliferation of mesangial cells. |
| Databáze: | OpenAIRE |
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