Role of placental inflammatory mediators and growth factors in patients with rheumatic diseases with a focus on systemic sclerosis

Autor: Véronique Ramoni, Fausta Beneventi, Giuseppina Ferrario, Stefania Cesari, Hanna Johnsson, Gerard J. Graham, Veronica Codullo, Carlomaurizio Montecucco, Giacomo Fiandrino, Francesca Motta, Stefania Rampello
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Chemokine
Fetal Membranes
Premature Rupture
CD3 Complex
chemokines
Systemic scleroderma
Chemokine receptor
0302 clinical medicine
Pre-Eclampsia
Pregnancy
Leukocytes
Lupus Erythematosus
Systemic
Pharmacology (medical)
skin and connective tissue diseases
Chemokine CCL5
AcademicSubjects/MED00360
biology
integumentary system
CD68
Hepatocyte Growth Factor
Gestational age
Clinical Science
Immunohistochemistry
medicine.anatomical_structure
Sjogren's Syndrome
Cytokines
Intercellular Signaling Peptides and Proteins
Premature Birth
Female
Receptors
Chemokine
medicine.symptom
Adult
HELLP Syndrome
placenta
inflammatory cells
Antigens
Differentiation
Myelomonocytic
Inflammation
CCL5
03 medical and health sciences
Rheumatology
Antigens
CD
Placenta
Rheumatic Diseases
medicine
Humans
Undifferentiated Connective Tissue Diseases
030203 arthritis & rheumatology
Scleroderma
Systemic
business.industry
medicine.disease
Antigens
CD20
Arthritis
Juvenile
CD11c Antigen
030104 developmental biology
Case-Control Studies
Immunology
biology.protein
business
SSc
Zdroj: Rheumatology (Oxford, England)
ISSN: 1462-0332
1462-0324
Popis: Objectives Pregnancy in SSc is burdened with an increased risk of obstetric complications. Little is known about the underlying placental alterations. This study aimed to better understand pathological changes and the role of inflammation in SSc placentas. Leucocyte infiltration, inflammatory mediators and atypical chemokine receptor 2 (ACKR2) expression in SSc placentas were compared with those in other rheumatic diseases (ORD) and healthy controls (HC). Methods A case–control study was conducted on eight pregnant SSc patients compared with 16 patients with ORD and 16 HC matched for gestational age. Clinical data were collected. Placentas were obtained for histopathological analysis and immunohistochemistry (CD3, CD20, CD11c, CD68, ACKR2). Samples from four SSc, eight ORD and eight HC were analysed by qPCR for ACKR2 expression and by multiplex assay for cytokines, chemokines and growth factors involved in angiogenesis and inflammation. Results The number of placental CD3, CD68 and CD11 cells was significantly higher in patients affected by rheumatic diseases (SSc+ORD) compared with HC. Hepatocyte growth factor was significantly increased in the group of rheumatic diseases patients (SSc+ORD) compared with HC, while chemokine (C-C motif) ligand 5 (CCL5) was significantly higher in SSc patients compared with ORD and HC. CCL5 levels directly correlated with the number of all local inflammatory cells and higher levels were associated with histological villitis. Conclusions Inflammatory alterations characterize placentas from rheumatic disease patients and could predispose to obstetric complications in these subjects.
Databáze: OpenAIRE
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