Association of Circulating miRNA Expression with Preeclampsia, Its Onset, and Severity
Autor: | Marian Grendar, Andrea Hornakova, Dusan Loderer, Veronika Holubekova, Marcela Nachajova, Terezia Pribulova, Zuzana Kolková, Pavol Zubor, Kamil Biringer, Imrich Zigo |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Angiogenesis miR-16-5p Clinical Biochemistry Physiology Disease Article Preeclampsia Pathogenesis preeclampsia 03 medical and health sciences miR-210-5p 0302 clinical medicine Downregulation and upregulation microRNA medicine miR-155-5p plasma lcsh:R5-920 Pregnancy 030219 obstetrics & reproductive medicine business.industry Trophoblast biomarkers medicine.disease 030104 developmental biology medicine.anatomical_structure miR-21-5p lcsh:Medicine (General) business |
Zdroj: | Diagnostics Volume 11 Issue 3 Diagnostics, Vol 11, Iss 476, p 476 (2021) |
ISSN: | 2075-4418 |
Popis: | MicroRNAs (miRNAs) are one of the important regulators of cellular functions fundamental for healthy pregnancy processes, including angiogenesis and differentiation of trophoblast cells, and their deregulation could be implicated in the pathogenesis of pregnancy complications, including preeclampsia (PE). The aim of this study was to assess the association of miRNA expression in plasma samples with PE, its onset, and severity. Our study enrolled 59 pregnant women, 27 in the preeclamptic study group and 32 in the control group with physiological pregnancy. Preeclamptic pregnancies were divided into subgroups based on the severity and onset of disease. Relative expression of miR-21-5p, miR-155-5p, miR-210-5p, miR-16-5p, and miR-650 isolated from plasma samples was analysed by quantitative real-time PCR and normalised to experimentally established reference genes. Our results revealed upregulation of miR-21-5p (1.16-fold change, p = 0.0015), miR-155-5p (1.62-fold change, p = 0.0005) in preeclamptic pregnancies, compared to controls. Overexpression of these two miRNAs was observed, especially in subgroups of severe and late-onset PE compared to healthy pregnancies. Although we hypothesised that the expression level of studied miRNAs could vary between PE subtypes (mild vs. severe, early onset vs. late-onset), no obvious differences were detected. In conclusion, our study could contribute to the large-scale studies for the identification of non-invasive biomarkers for PE detection to improve outcomes for women and their new-borns. |
Databáze: | OpenAIRE |
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