UVB-induced leucocyte trafficking in the epidermis of photosensitive lupus erythematosus patients: Normal depletion of Langerhans cells
| Autor: | Artiena Soe Janssens, Rein Willemze, Frank R. de Gruijl, Stan Pavel, Coby Out-Luiting, Eileen E L O Lashley |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Time Factors Langerhans cell Adolescent Light Ultraviolet Rays Dermatology Biochemistry Subacute cutaneous lupus erythematosus Pathogenesis Photosensitivity Biopsy Leukocytes Lupus Erythematosus Cutaneous medicine Humans Lupus Erythematosus Systemic Photosensitivity Disorders skin and connective tissue diseases Molecular Biology Skin Lupus erythematosus integumentary system medicine.diagnostic_test Epidermis (botany) business.industry Middle Aged medicine.disease Immunohistochemistry Connective tissue disease medicine.anatomical_structure Langerhans Cells Immunology Female Epidermis business |
| Zdroj: | Experimental Dermatology. 14:138-142 |
| ISSN: | 1600-0625 0906-6705 |
| DOI: | 10.1111/j.0906-6705.2005.00279.x |
| Popis: | Background: The pathogenic mechanisms of UV-induced skin lesions of lupus erythematosus (LE) are unknown. In a recent study of pathogenic mechanisms of polymorphic light eruption (PLE), significantly more Langerhans cells (LCs) persisted in the epidermis after UVB overexposure than in healthy individuals. Interestingly, the same phenomenon was observed in one subacute cutaneous lupus erythematosus (SCLE) patient. It could therefore be hypothesized that both photodermatoses share a common pathogenic mechanism of photosensitivity. In the present study, we tested this hypothesis by investigating leucocyte trafficking in the initial phase of cutaneous LE after intense UVB exposure. Methods: In 22 photosensitive LE patients (12 chronic discoid lupus erythematosus, seven systemic lupus erythematosus and three SCLE) and nine age/sex-matched controls, uninvolved buttock skin was exposed to six minimal erythemal dose (MED) UVB radiation. Subsequently, biopsies were taken after 24, 48 and 72 h, and one control biopsy was taken from unirradiated skin. Skin sections were stained for the presence of LCs, neutrophils and macrophages. Areal percentages of positively stained cells within the epidermis were quantified and compared between the patients and controls. Results: A gradual decrease of epidermal LCs and a gradual increase of epidermal neutrophils and macrophages at several timepoints after six MED irradiation was observed equally in both LE patients and controls. Conclusion: Immunohistopathology of irradiated uninvolved skin of photosensitive LE patients did not reveal the same pathologic trafficking of LCs and neutrophils as described for PLE patients. We conclude that different mechanisms are operative in the pathogenesis of PLE and photosensitive LE. |
| Databáze: | OpenAIRE |
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